Vasotocin and oxytocin modulation of the endocrine and behavioral response to an aggressive challenge in male Siamese fighting fish

Abstract

Aggressive behavior is an adaptive trait present across all taxa. However, the neuroendocrine mechanisms regulating it, particularly in fish, are not well understood. Oxytocin (OXT) and arginine vasotocin (VT) are known modulators of aggression, but their actions remain controversial. This study tested the possible modulation of endocrine and behavioral responses to an aggression challenge by these nonapeptides in Siamese fighting fish, Betta splendens, a species known for its intrinsic aggressiveness. Male B. splendens were injected with different dosages of either Manning compound or L-368,899, VT and OXT receptor antagonists respectively, and were exposed to a mirror challenge for 30 min. While all fish displayed high levels of aggression toward their mirror image, no differences were observed between control-injected and treatment fish. However, blocking VT inhibited the post-fight increase in plasma levels of the androgen 11-ketotestosterone (KT). To further investigate this result, testis tissue from males was incubated with and without VT and Manning compound, and KT levels were measured after 180 min. Results showed a direct effect of VT on in vitro KT secretion, indicating the presence of VT receptors in the testes of this species. Overall, the study does not support a modulatory role of VT or OXT in aggressive behavior, although VT might be implicated in the regulation of peripheral androgen response to aggression in B. splendens.

Keywords: 11-ketotestosterone; Aggression; Arginine vasotocin; Betta splendens; Oxytocin.