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. 2025 May;136(5):e70034.
doi: 10.1111/bcpt.70034.

Exploring Retene's Tumour-Initiating Potential: Integrating Computational and Experimental Approaches

Francisco Carlos da Silva Junior  1   2   3 , Thiago Pires Cláudio  4 , Ricardo Luiz Cavalcanti de Albuquerque-Júnior  5 , Silvia Regina Batistuzzo de Medeiros  1   2
Affiliations

Exploring Retene's Tumour-Initiating Potential: Integrating Computational and Experimental Approaches

Francisco Carlos da Silva Junior et al. Basic Clin Pharmacol Toxicol. 2025 May.
No abstract available

Keywords: Swiss albino mice; bioinformatic tools; carcinogenicity; chemical skin‐carcinogenesis; retene.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
(A) Gross changes observed in the of the experimental animals. Skin wrinkling (WR) and thickening (TH), desquamative hyperkeratosis (DH), large (black arrow) and small (white arrow) skin ulcerations, leukoplastic plaques (LP), small isolated verrucous polypoid papules (area delimited by black line), as well as multiple and sometimes confluent, irregular polypoid nodules (area delimited by red line). (B) Assessment of the average index of chemically induced skin lesions (InL) in mice. Data are expressed as mean ± standard deviation (SD). Different letters above each column mean significantly different means (p < 0.05), whereas equal letters represent statistically similar means (p > 0.05) (ANOVA, followed by Bonferroni's multiple comparison test).
FIGURE 2
FIGURE 2
Photomicrographs of HE‐stained histological slides representative of the main histological changes observed in the skin of the experimental groups. (A–C) Areas of ulceration and marginal mild epithelial acanthosis in the SC group. (D,E) Group RET 10 μM shows focal areas of blunt papillomatosis with superficial epithelial desquamation and foci of epithelial atrophy. (F,G) Group RET 20 and (H,I) RET 30 μM showing higher dermal papillary projections covered by squamous epithelium with focal areas of mild dysplasia. In group RET 40 μM (J–L), high papillary projections covered by proliferative dysplastic epithelium. Scanty epithelial nests apparently ‘detached’ from the lining epithelium (pseudo carcinomatous hyperplasia) are observed in an intensely inflamed dermal connective tissue. Areas of papillary projections (M,N), covered by dysplastic hyperkeratinized squamous epithelium with keratin plugs formation; and (O,P) areas of development of markedly exophytic lesions with foci of pseudocarcinomatous hyperplasia. (Q,R) Invasive carcinoma deriving from the epidermal lining comprised of well‐differentiated neoplastic squamous cells; and (S,T) Noninvasive papillomas, with high mitotic index, observed in the DMBA‐treated animals.
See this image and copyright information in PMC

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