Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun;104(6):105100.
doi: 10.1016/j.psj.2025.105100. Epub 2025 Mar 25.

Split-feeding as a sustainable feeding strategy for improving egg production and quality, nutrient digestibility, and environmental impact in laying hens

Affiliations

Split-feeding as a sustainable feeding strategy for improving egg production and quality, nutrient digestibility, and environmental impact in laying hens

Hyung Suk Hwang et al. Poult Sci. 2025 Jun.

Abstract

This study evaluated the effects of split feeding on production performance, egg quality, nutrient digestibility, odor emissions, and economic benefits in laying hens. A total of 468 Hy-Line Brown hens, aged 63 weeks, were divided into three dietary treatments over 13 weeks. The control group received a single basal diet, while two split-feeding groups (TRT1 and TRT2) received diets differing in nutrient composition between the morning and afternoon. TRT1 and TRT2 contained 10 % and 15 % less crude protein (CP), respectively, compared to CON. TRT1 maintained the same levels of metabolizable energy, standardized ileal digestibility amino acids, calcium, and phosphorus as CON, while TRT2 had 5 % lower metabolizable energy, standardized ileal digestibility amino acids, and calcium, and 15 % lower phosphate. Results showed that TRT1 reduced the incidence of downgraded eggs by 19.3 % in the later phase of the experiment (9-12 weeks) compared to CON (P = 0.025, 0.043, 0.022, 0.011). Both TRT1 and TRT2 reduced diet costs by 6 % and 15 %, respectively. Additionally, split feeding improved nutrient digestibility, reduced harmful gas emissions, and resulted in a 4.5 % feed cost savings per kilogram of salable egg production compared to CON. These findings suggest that split feeding (TRT1) could enhance egg quality, reduce environmental impact, and contribute to the sustainability of poultry farming.

Keywords: Calcium availability; Egg quality; Layer; Split feeding.

PubMed Disclaimer

Conflict of interest statement

Disclosures The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Similar articles

Cited by

  • Split feeding for laying hens: a step beyond precision nutrition.
    do Nascimento CH, de Lima AV, de Souza PEL, de Souza Filho DF, Santos Silva RD, Rodrigues AB, Neto RDCL, de Lima MR, Guerra RR, Ribeiro AG, Carvalho LRRA, Costa FGP. do Nascimento CH, et al. Poult Sci. 2025 Jul;104(7):105158. doi: 10.1016/j.psj.2025.105158. Epub 2025 Apr 13. Poult Sci. 2025. PMID: 40273685 Free PMC article. Review.

References

    1. AOAC . 2000. Official Methods of Analysis. 17th.
    1. Akter N., Dao T.H., Crowley T.M., Moss A.F. Optimization of split feeding strategy for laying hens through a response surface model. Animals. 2025;15(5):750. - PMC - PubMed
    1. Araújo J.A., Silva J.H.V., Costa F.G.P., Sousa J.M.B., Givisiez P.E.N., Sakomura N.K. Effect of the levels of calcium and particle size of limestone on laying hens. Rev. Bras. Zootec. 2011;40(5):997–1005.
    1. De Reu K., Rodenburg T.B., Grijspeerdt K., Messens W., Heyndrickx M., Tuyttens F.A.M., Sonck B., Zoons J., Herman L. Bacteriological contamination, dirt, and cracks of eggshells in furnished cages and noncage systems for laying hens: an international on-farm comparison. Poult. Sci. 2009;88(11):2442–2448. - PubMed
    1. De Vries J.W., Groenestein C.M., De Boer I.J.M. Environmental consequences of processing manure to produce mineral fertilizer and bio-energy. J. Environ. Manage. 2015;102:183–192. - PubMed