Methylation of immature small ribosomal subunits by methyltransferases conferring aminoglycoside resistance

Abstract

Aminoglycosides are broad-spectrum antibiotics critical to clinical treatment, but the emergence of bacterial resistance, particularly through 16S rRNA methyltransferases, has compromised their efficacy. These enzymes, originally discovered in natural aminoglycoside producers, confer resistance by methylating nucleotides G1405 and A1408 in 16S rRNA, blocking antibiotic binding to the ribosome. This study investigated the binding affinities and methylation activities of 16S rRNA methyltransferases KamB, NpmA, RmtA, RmtC, and Sgm with immature 30S ribosomal subunits from E. coli strains lacking RimM and YjeQ ribosomal assembly factors. Binding affinities to mature 30S ribosomal subunits and immature 30S assembly forms isolated from ΔyjeQ and ΔrimM strains were determined by microscale thermophoresis and interactions were further validated with in vitro pull-down assays. Methylation of immature 30S subunits was examined with primer extension on 16S rRNA extracted from methylation assays in vitro and from cells with immature 30S subunits expressing 16S rRNA methyltransferases in vivo, showing successful methylation of target nucleotides in both experimental systems. The results reveal that aminoglycoside resistance methyltransferases are capable to bind and modify late-stage immature 30S ribosomal subunits pointing to possibility that the resistance to aminoglycoside antibiotics is installed and established before the full maturation of ribosomal 30S subunit.

Keywords: 16S rRNA methyltransferases; Aminoglycoside resistance; Ribosome assembly factors; RimM; YjeQ.