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. 2025 May 8;14(5):e0132824.
doi: 10.1128/mra.01328-24. Epub 2025 Apr 14.

Sequence analysis of two F1 mycobacteriophages, Deb65 and DocMcStuffins

Marcus O Royster  1 , Victoria Figgins  1 , Vera Pande  1 , Jason D Robinson  1 , Deeka S Abdi  1 , Ali Amin  1 , Zephaniah Ansah  1 , Ethan W Bomersheim  1 , Gianna Dunn  1 , Ali A Elfaki  1 , Jordyn Foulk  1 , Kate C Ingle  1 , Avi D Lavu  1 , Ved Pande  1 , Priya T Shan  1 , Marie P Smithbey  1 , Gunnar R Ternstrom  1 , Olivia S Trager  1 , David A Washington  1 , Monica Xu  1 , Margaret S Saha  1
Affiliations

Sequence analysis of two F1 mycobacteriophages, Deb65 and DocMcStuffins

Marcus O Royster et al. Microbiol Resour Announc. .

Abstract

Isolated from wetland soil, Deb65 and DocMcStuffins are bacteriophages with a siphoviral morphology that infect Mycobacterium smegmatis. Deb65 and DocMcStuffins encode 97 and 91 putative genes, 41 of which are shared. Based on gene content similarity to actinobacteriophages more broadly, both phages are assigned to subcluster F1.

Keywords: Mycobacterium smegmatis; mycobacteriophage.

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Conflict of interest statement

The authors declare no conflict of interest

Figures

Fig 1
Fig 1
(a) Negative stain (uranyl acetate, 1%) transmission electron microscopy of DocMcStuffins revealing siphoviral morphology. Scale bar is 50 nm. (b) Negative stain (uranyl acetate, 1%) transmission electron microscopy of Deb65 revealing siphoviral morphology. Scale bar is 50 nm. (c) Plaque image of DocMcStuffins showing clear plaques. Scale bar is 6.125 mm. (d) Plaque image of Deb65 showing clear plaques. Scale bar is 6.125 mm. (e) Phamerator comparative alignment of Deb65 and DocMcStuffins.
See this image and copyright information in PMC

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