Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Feb;129(2):789-95.
doi: 10.1128/jb.129.2.789-795.1977.

Some properties of a membrane-deoxyribonucleic acid complex isolated from Bacillus subtilis

J M Harmon, H W Taber

Some properties of a membrane-deoxyribonucleic acid complex isolated from Bacillus subtilis

J M Harmon et al. J Bacteriol. 1977 Feb.

Abstract

Membrane-deoxyribonucleic acid (DNA) complexes were isolated from Bacillus subtilis by affinity for magnesium-Sarkosyl crystals. These complexes (M-bands) contained greater than 80% of the total cellular DNA; little of the remaining portion could be recovered in a secondary isolation. Isotopic labeling of the origin of replication showed this region of the chromosome to be closely associated with the cell membrane. Interruption of protein or DNA synthesis did not result in detachment of the chromosome from the membrane. Interruption of ribonucleic acid synthesis by rifampin resulted in a decreased ability to isolate DNA in the M-band. Analysis of attachment of the chromosome to membrane during the cell and replication cycles indicated that the chromosome is not released from the membrane at any time during the cell cycle.

PubMed Disclaimer

References

    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. Ann Inst Pasteur (Paris). 1964 Sep;107:384-400 - PubMed
    1. Nat New Biol. 1973 Aug 15;244(137):204-6 - PubMed
    1. Nature. 1974 Sep 20;251(5472):252-4 - PubMed
    1. J Bacteriol. 1973 Dec;116(3):1364-74 - PubMed

Publication types

LinkOut - more resources