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Review
. 2025 Mar 21;26(7):2828.
doi: 10.3390/ijms26072828.

Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation

Xiaogeng Yang  1   2 , Honghong He  1   2 , Peng Wang  3 , Yaying Wang  1   2 , Linlin Wang  4 , Falong Yang  1   2 , Jian Li  1   2 , Huizhu Zhang  1   2
Affiliations
Review

Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation

Xiaogeng Yang et al. Int J Mol Sci. .

Abstract

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate target gene expression in many eukaryotes. MiRNAs are essential for post-transcriptional regulation, influencing various biological functions, including oocyte growth and maturation, fertilization, early embryo development, and implantation. In recent decades, numerous studies have identified a substantial number of miRNAs associated with mammalian oocyte maturation and early embryo development, utilizing methods such as small RNA sequencing and modulating miRNA expression through overexpression or inhibition. In this review, we introduce the biosynthesis of miRNAs and their regulatory roles in germ cells, summarizing the expression patterns and post-transcriptional regulation of miRNAs during bovine oocyte maturation and early embryo development, as well as their potential application in bovine assisted reproductive technology (ART).

Keywords: ART; bovine; embryo development; miRNA; oocyte maturation.

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Conflict of interest statement

The authors claim no conflicts of interest.

Figures

Figure 1
Figure 1
Canonical pathway of miRNAs biosynthesis. Within the nucleus, miRNAs are initially transcribed by RNA polymerase II into pri-miRNAs. The enzyme Drosha forms microprocessor complexes with DGCR8, cleaving pri-miRNA to produce pre-miRNAs. The complexes cleave the pri-miRNA into pre-miRNAs, which are transported to the cytoplasm by Exportin-5. In the cytoplasm, the Dicer complex cleaves the pre-miRNAs, leading to mature miRNAs. The mature miRNAs then interfere with the translation mechanisms by binding to complementary target sequences in the 3′-untranslated region (3′-UTR) of the mRNA, leading to translation inhibition or mRNA degradation.
Figure 2
Figure 2
MiRNAs regulate bovine oocyte maturation and preimplantation embryo development. GV: germinal vesicle; MI: metaphase I; MII: metaphase II.
See this image and copyright information in PMC

References

    1. Ferré L.B., Kjelland M.E., Strøbech L.B., Hyttel P., Mermillod P., Ross P.J. Review: Recent advances in bovine in vitro embryo production: Reproductive biotechnology history and methods. Animal. 2020;14:991–1004. doi: 10.1017/S1751731119002775. - DOI - PubMed
    1. Lee K.B., Wee G., Zhang K., Folger J.K., Knott J.G., Smith G.W. Functional role of the bovine oocyte-specific protein JY-1 in meiotic maturation, cumulus expansion, and subsequent embryonic development. Biol. Reprod. 2014;90:69. doi: 10.1095/biolreprod.113.115071. - DOI - PubMed
    1. Liang L., He X. A narrative review of microRNA therapeutics: Understanding the future of microRNA research. Precis. Cancer Med. 2021;4:33. doi: 10.21037/pcm-21-28. - DOI
    1. Diener C., Keller A., Meese E. Emerging concepts of miRNA therapeutics: From cells to clinic. Trends Genet. 2022;38:613–626. doi: 10.1016/j.tig.2022.02.006. - DOI - PubMed
    1. Kropp J., Salih S.M., Khatib H. Expression of microRNAs in bovine and human pre-implantation embryo culture media. Front. Genet. 2014;5:91. doi: 10.3389/fgene.2014.00091. - DOI - PMC - PubMed

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