Synthesis of an experimental gel containing cannabidiol oil and evaluation of effects on bleached bovine enamel

Abstract

To obtain and characterize a gel containing cannabidiol (CBD) oil using Fourier transform infrared spectroscopy, high-performance liquid chromatography (HPLC), thermogravimetry, and differential scanning calorimetry, and to evaluate its effects on color change (∆E00), surface roughness (Ra), Knoop microhardness (KHN), ultrastructure, and mineral content in bovine enamel after bleaching with 35% hydrogen peroxide (HP35%). For HPLC, diluted CBD oil was used, UV spectra were obtained in the range of 4000-650 cm - 1 with a resolution of 4 cm - 1, and for thermal profile determination, CBD oil and the gel were heated from 20 to 300 °C. To evaluate the effects on bovine dental enamel, 40 specimens were distributed into 4 groups (n = 10): control-untreated; HP35%-bleaching with HP35%; KF2-5% potassium nitrate and 2% sodium fluoride + HP35%; CBD-CBD gel + HP35%. The bleaching treatment was performed in 3 sessions with a 72 h interval. Desensitizers were applied for 10 min prior to bleaching. Results were evaluated by one-way ANOVA and Tukey test (p < 0.05). Analyses demonstrated a satisfactory profile for the use of CBD gel. All treated groups showed significant bleaching effect, and there was no difference among them regarding ∆E00, Ra, and KHN. The experimental CBD gel proved to be safe when used prior to the bleaching agent, as it did not influence the effectiveness of bleaching, surface roughness, microhardness, and mineral content of bovine dental enamel.

Keywords: Cannabidiol; Dental enamel; Fourier transform infrared spectroscopy; Liquid chromatography; Surface properties; Tooth bleaching.