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. 2025 Sep;69(18):e70009.
doi: 10.1002/mnfr.70009. Epub 2025 Apr 21.

Dietary Total Antioxidant Capacity Can Modify the Effects of Apo-B Polymorphisms (Ins/Del and EcoRI) on Lipid Profiles and Atherogenic Indices Between Diabetic Patients

Faezeh Abaj  1   2 , Masoumeh Rafiee  3 , Ronak Nikbazm  4 , Ehsan Alvandi  5 , Fariba Koohdani  6
Affiliations

Dietary Total Antioxidant Capacity Can Modify the Effects of Apo-B Polymorphisms (Ins/Del and EcoRI) on Lipid Profiles and Atherogenic Indices Between Diabetic Patients

Faezeh Abaj et al. Mol Nutr Food Res. 2025 Sep.

Abstract

We aimed to study the role of Apolipoprotein B (Apo-B) polymorphisms (Ins/Del and EcoRI) and genotype interaction on lipid profiles and atherogenic indices in response to changes in dietary total antioxidant capacity (DTAC) of diet. This cross-sectional study consisted of 700 diabetic patients. Biochemical markers including total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), superoxide dismutase (SOD), C-reactive protein (CRP), total antioxidant capacity (TAC), interlukin-18 (IL-18), and Prostaglandin F2α (PGF2α) were measured based on standard protocols. Genotyping of the Apo-B polymorphisms was conducted by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Subjects with Ins/Ins genotype with higher DTAC intake had lower TG, AIP, and AC compared to Del-allele carriers. Moreover, A-allele carriers (EcoRI) with a higher median intake of DTAC had lower body mass index (BMI) and waist circumference (WC) compared to GG homozygotes. For combined genotypes, the EcoRI only variant (Ins/Ins and AA + AG) with higher DTAC intake had lower BMI and WC. Moreover, Ins/Del only variant (Ins/del + del/del and GG) with more adherence to DTAC had higher TG and AIP. Our study showed that Apo-B polymorphisms interact with the antioxidant capacity of diet to ameliorate the risk of cardio-metabolic diseases, especially atherosclerosis in the A carriers of EcoR1 and Ins/Ins homozygous of Ins/Del polymorphism.

Keywords: Apo‐B polymorphism; T2DM; dietary total antioxidant capacity (DTAC); nutrigenetic.

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