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. 2025 Apr 14:18:1881-1893.
doi: 10.2147/IDR.S509957. eCollection 2025.

Developing a Urinary Catheter with Anti-Biofilm Coated Surface Using Phyto-Assisted Synthesis of Zinc Oxide Nanoparticles

Reham M Goda #  1 , Ibrahim A Maghrabi  2 , Mohamed F El-Badawy #  3 , Ahmed M Kabel  4 , Alaa A Omar  5 , Rasha M El-Morsi  1 , Heba A Ramadan  1 , Mohamed M Shohayeb  1
Affiliations

Developing a Urinary Catheter with Anti-Biofilm Coated Surface Using Phyto-Assisted Synthesis of Zinc Oxide Nanoparticles

Reham M Goda et al. Infect Drug Resist. .

Abstract

Background: Biofilm-related infections represent one of the major challenging health problems that enhances antimicrobial resistance with subsequent treatment failure of catheter-associated urinary tract infections (CAUTIs).

Aim: This study aimed to employ and comprehensively characterize the use of nanoparticles to inhibit bacterial biofilm formation. Zinc oxide nanoparticles (ZnO-NPs) are considered one of the most important biofilm inhibitors.

Methods: The current study aimed to characterize the influence of the bioreductive green synthesis of ZnO-NPs using pomegranate peel extract on bacterial colonization to protect against urinary catheter infections. ZnO-NPs were investigated for their physicochemical properties using UV, FTIR, Dynamic light scattering, and TEM. Catheters were coated with ZnO-NPs using Pistacia lentiscus (mastic), and the slow release of free zinc ions (Zn+2) from, the ZnO-NPs-coated catheters, was evaluated using the ICP-AES technique.

Results: The current study revealed that catheter coated by ZnO-NPs exhibited a sustained antibiofilm activity against biofilm-forming and antibiotic-resistant clinical isolates of Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa strains.

Conclusion: The present study supports the efficiency of ZnO-NPs as a good candidate for prevention of biofilm formation.

Keywords: bacterial colonization; biofilm; catheter associated infection; urinary catheters; zinc oxide nanoparticles.

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Conflict of interest statement

The authors declare no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Schematic illustration of ZnO-NPs green synthesis using pomegranate peel extract.
Figure 2
Figure 2
UV-Vis spectral analysis of synthesized ZnO-NPs.
Figure 3
Figure 3
Particle size distribution (average mean diameter) measured by dynamic light scattering (DLS) for the synthesized ZnO-NPs (A). Zeta potential of the synthesized ZnO-NPs (B).
Figure 4
Figure 4
TEM micrographs of the synthesized ZnO-NP.
Figure 5
Figure 5
FTIR spectra of pomegranate extract (black), zinc sulphate ZnSO4.7H2O (blue) and ZnO-NPs (red).
Figure 6
Figure 6
The inhibitory effect of ZnO-NPs on Klebsiella pneumoniae (A) and Staphylococcus epidermidis (B) by cup-plate method. The inhibitory effect of catheter pieces coated by f ZnO-NPs on K. pneumoniae (C) and S. epidermidis (D).
Figure 7
Figure 7
Scanning electron micrographs (SEM) for bacterial colonization of control catheters (A and C) and catheters varnished with mastic and ZnO-NPs (B and D), after 48h impregnation in tryptic soya broth inoculated with 105 CFU mL-1 of Staphylococcus aureus (A and B) and Klebsiella pneumoniae (C and D).
Figure 8
Figure 8
The release of Zn2+ from ZnO-NPs-coated catheters.
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References

    1. Ifeanyichukwu UL, Fayemi OE, Ateba CN. Green synthesis of zinc oxide nanoparticles from pomegranate (Punica granatum) extracts and characterization of their antibacterial activity. Molecules. 2020;25(19):4521. doi:10.3390/molecules25194521 - DOI - PMC - PubMed
    1. Shakerimoghaddam A, Ghaemi EA, Jamalli A. Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenic Escherichia coli. Iran J Basic Med Sci. 2017;20(4):451. doi:10.22038/IJBMS.2017.8589 - DOI - PMC - PubMed
    1. Ozdal M, Gurkok S. Recent advances in nanoparticles as antibacterial agent. ADMET and DMPK. 2022;10(2):115–129. doi:10.5599/admet.1172 - DOI - PMC - PubMed
    1. Ukidave VV, Ingale LT. Green synthesis of zinc oxide nanoparticles from Coriandrum sativum and their use as fertilizer on Bengal gram, Turkish gram, and green gram plant growth. Inter J Agron. 2022;2022(1):8310038. doi:10.1155/2022/8310038 - DOI
    1. Faisal S, Jan H, Shah SA, et al. Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous fruit extracts of Myristica fragrans: their characterizations and biological and environmental applications. ACS Omega. 2021;6(14):9709–9722. doi:10.1021/acsomega.1c00310 - DOI - PMC - PubMed

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