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. 2025:2918:221-228.
doi: 10.1007/978-1-0716-4482-9_18.

In-Gel Zymography of Amyotrophic Lateral Sclerosis-Associated Variants of Superoxide Dismutase-1

Jeremy S Lum  1 , Mikayla L Brown  2 , Siobhan C Suters  2 , Justin J Yerbury  2 , Luke McAlary  3
Affiliations

In-Gel Zymography of Amyotrophic Lateral Sclerosis-Associated Variants of Superoxide Dismutase-1

Jeremy S Lum et al. Methods Mol Biol. 2025.

Abstract

In-gel zymography allows the separation of protein via electrophoresis and subsequent measurement of enzymatic activity of enzymes from biological mixtures. The antioxidant enzyme superoxide dismutase 1 (SOD1) is an important cytosolic oxygen radical scavenging enzyme that has been implicated in a range of pathologies, including cancer, metabolic and neurodegenerative diseases, most notably amyotrophic lateral sclerosis (ALS). Here, we describe a method to detect and compare SOD1 activity from both cell and tissue samples. This method can be utilized to compare differences between ALS-associated SOD1 genetic variants and pharmacologically treated biological samples.

Keywords: Amyotrophic lateral sclerosis; Electrophoresis; In-gel zymography; SOD1; Superoxide dismutase-1.

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References

    1. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287 - DOI - PubMed
    1. Grudkowska M, Lisik P, Rybka K (2013) Two-dimensional zymography in detection of proteolytic enzymes in wheat leaves. Acta Physiol Plant 35:3477–3482 - DOI
    1. Lum JS, Brown ML, Farrawell NE et al (2021) CuATSM improves motor function and extends survival but is not tolerated at a high dose in SOD1G93A mice with a C57BL/6 background. Sci Rep 11:19392 - DOI - PubMed - PMC
    1. McAlary L, Shephard VK, Wright GSA et al (2022) A copper chaperone-mimetic polytherapy for SOD1-associated amyotrophic lateral sclerosis. J Biol Chem 298:101612 - DOI - PubMed - PMC
    1. Chen J-R, Liao C-W, Mao SJT et al (2001) A simple technique for the simultaneous determination of molecular weight and activity of superoxide dismutase using SDS–PAGE. J Biochem Biophys Methods 47:233–237 - DOI - PubMed

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