Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul;175(3):339-358.
doi: 10.1111/imm.13936. Epub 2025 Apr 23.

Development of a Novel Multi-Epitope Vaccine Against Streptococcus anginosus Infection via Reverse Vaccinology Approach

Linglan Xu  1   2   3 , Nan Xie  1 , Yiqing Liu  1 , Hongmei Tang  1 , Tian Li  4 , Jiaofeng Peng  2 , Ranhui Li  1   3
Affiliations

Development of a Novel Multi-Epitope Vaccine Against Streptococcus anginosus Infection via Reverse Vaccinology Approach

Linglan Xu et al. Immunology. 2025 Jul.

Abstract

Streptococcus anginosus is an opportunistic pathogen known for its capability to cause a broad range of infections, posing a significant and growing global health concern. Alongside enhancing diagnostic capabilities and bolstering public health initiatives, developing a safe and effective vaccine represents a promising strategy to tackle this health challenge. In this paper, we employed an array of bioinformatics tools to engineer a subunit vaccine that exhibits high immunogenicity against S. anginosus. After constructing the multi-epitope vaccine, we subsequently predicted its secondary and tertiary protein structures. After refining and validating the modelled structure, we utilised advanced computational approaches, including molecular docking and dynamic simulations, to evaluate the binding affinity, compatibility, and stability of the vaccine-adjuvant complexes. Eventually, in silico cloning was conducted to optimise protein expression and production. The multi-epitope subunit vaccine we developed showed properties in antigenicity and immunity theoretically. The computational study revealed that this vaccine demonstrates significant efficacy against S. anginosus.

Keywords: Streptococcus anginosus; immune simulation; molecular dynamics simulation; reverse vaccinology.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. C. P. Andam and W. P. Hanage, “Mechanisms of Genome Evolution of Streptococcus,” Infection, Genetics and Evolution 33 (July 2015): 334–342.
    1. O. Oppegaard, M. Glambek, D. H. Skutlaberg, S. Skrede, A. Sivertsen, and B. R. Kittang, “Streptococcus dysgalactiae Bloodstream Infections, Norway, 1999–2021,” Emerging Infectious Diseases 29, no. 2 (2023): 260–267.
    1. M. Yildirim‐Aksoy, B. H. Beck, and D. Zhang, “Examining the Interplay Between Streptococcus agalactiae, the Biopolymer Chitin and Its Derivative,” MicrobiologyOpen 8, no. 5 (May 2019): e00733.
    1. H. D. López‐Santacruz, A. López‐López, A. Revilla‐Guarinos, et al., “Streptococcus dentisani is a Common Inhabitant of the Oral Microbiota Worldwide and is Found at Higher Levels in Caries‐Free Individuals,” International Microbiology 24, no. 4 (November 2021): 619–629.
    1. J. E. Clarridge, S. Attorri, D. M. Musher, J. Hebert, and S. Dunbar, “Streptococcus Intermedius, Streptococcus constellatus, and Streptococcus Anginosus (“Streptococcus Milleri Group”) are of Different Clinical Importance and are not Equally Associated with Abscess,” Clinical Infectious Diseases 32, no. 10 (May 2001): 1511–1515.

LinkOut - more resources