doi: 10.1016/0006-291x(85)90373-0.
Isolation and partial characterization of a novel basement membrane collagen
- PMID: 4026821
- DOI: 10.1016/0006-291x(85)90373-0
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Isolation and partial characterization of a novel basement membrane collagen
Biochem Biophys Res Commun.
.
Abstract
A guanidine-HCl extraction of lens capsule basement membrane dissolves collagenous material. This material was fractionated on an Agarose A-5M column. Fractions 1, 2 and 3 were further purified and partially characterized immunochemically and by amino acid analysis. Fraction 3 has a molecular weight of 55,000 when compared with collagen type I standard. The CNBr peptide pattern and composition of fraction 3 are different from those of alpha 1 (IV) 95K and alpha 2 (IV) 95K chains. The results described suggest the presence of a new chain in lens capsule basement membrane.
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