An evaluation of indirect immunofluorescence in the serological diagnosis of Nosema cuniculi infection

Abstract

An indirect immunofluorescence test for serum antibodies to Nosema cuniculi was evaluated in rabbits by comparison with established histopathological methods of diagnosis. Cell-culture isolation procedures and immunofluorescence and histological techniques designed to detect the parasite in urine or tissues were used to confirm the diagnosis. The serological diagnosis showed excellent correlation with presence of brain lesions characteristic of nosematosis, and N cuniculi were detected in 63 per cent of seropositive rabbits but in no seronegatives. Serum cross-reactivity tests between N cuniculi and Toxoplasma gondii showed that these two protozoa are antigenically distinct. The technique offers a simple, sensitive and reliable procedure for diagnosis of nosematosis in a living animal.