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. 2025 Mar 29;16(4):395.
doi: 10.3390/genes16040395.

Association Analysis of ENPP1 Tissue Expression, Polymorphism, and Growth Traits in Xiangsu Pigs

Jiaqi Chen  1   2 , Jiajin Huang  1   2 , Houqiang Xu  1   2
Affiliations

Association Analysis of ENPP1 Tissue Expression, Polymorphism, and Growth Traits in Xiangsu Pigs

Jiaqi Chen et al. Genes (Basel). .

Abstract

Background: Pigs are vital agricultural animals, with growth traits serving as key indicators of their quality. Methods: In this study, we examined the mRNA expression of ENPP1 as a candidate gene in heart, liver, spleen, lungs, and kidneys at 3 days and 6 months of age by real-time polymerase chain reaction method and single-nucleotide polymorphism (SNP) loci in 165 Xiangsu pigs by Sanger sequencing. Results: The expression of ENPP1 in different tissues of Xiangsu pigs at different stages was significantly different, and it had high conservation in different species.. Sequence alignment with reference data identified five SNP sites: g.64275T→C and g.64429G→A in intron 19, g.64850T→C and g.64911G→A in intron 20, and g.64527T→C in exon 20. Association analysis revealed that g.64275T→C, g.64429G→A, and g.64527T→C significantly influence the growth performance of Xiangsu pigs (p < 0.05). Conclusions: These findings suggest that ENPP1 polymorphisms are closely associated with growth traits in Xiangsu pigs and may provide valuable insights for molecular breeding of this breed.

Keywords: ENPP1; SNPs; Xiangsu hybrid pig; growth properties.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Expression level of ENPP1 in Xiangsu pigs. (A) Expression level of ENPP1 in tissues of 3-day-old Xiangsu pigs; (B) expression level of ENPP1 in tissues of 6-month-old Xiangsu pigs. * Indicating significant differences (p-value < 0.05); (C): Tissue expression of ENPP1 in different growth stages of Xiangsu pigs was different. ** different capital letters indicate extremely significant differences (p-value < 0.01).
Figure 2
Figure 2
SNP loci of ENPP1 gene in Xiangsu pig. (A) g.64275 T→C, (B) g.64429G→A, (C) g.64527T→C, (D) g.64850T→C, (E) g.64911G→A.
Figure 3
Figure 3
Evolutionary tree of the ENPP1 system for 10 species (Left) and motif structure analysis (Right).
Figure 4
Figure 4
The motif sequence of ENPP1 in 10 species.
Figure 5
Figure 5
Distribution area of growth data for 165 Xiangsu pigs.
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