Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Mar;232(3):H341-7.
doi: 10.1152/ajpheart.1977.232.3.H341.

Bone extraction and blood clearance of diphosphonate in the dog

S P Hughes, D R Davies, J B Bassingthwaighte, F G Knox, P J Kelly

Bone extraction and blood clearance of diphosphonate in the dog

S P Hughes et al. Am J Physiol. 1977 Mar.

Abstract

The transcapillary extraction of diphosphonate, as [99mTc]EHDP, a substance used in bone scanning and for management of certain metabolic bone diseases, has been examined. The maximum instantaneous extraction for [99mTc]EHDP was 0.27 +/- 0.05 (mean +/- SD, N = 10) and the net extraction at 5 min was 0.18 +/- 0.05 (N = 10). The permeability ratio of [99mTc]EHDP to the freely diffusible compound, sucrose, using the formula PS = -Fs loge (1 - Emax), was 0.71. This is similar to the ratio of diffusion coefficients of EHDP to sucrose, which is estimated to be 0.78. These results suggest that the mechanism by which [99mTc]EHDP passes through the capillaries in bone is passive diffusion. Tissue level estimations of EHDP confirm a rapid blood clearance associated with an increase in the rate of urinary excretion; the level of [99mTc]EHDP in bone, however, remains constant. The fractional excretion of [99mTc]EHDP was 27.3 +/- 2.0% in control dogs and was unchanged by thyroparathyroidectomy and subsequent infusion of parathyroid hormone.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Examples of outflow dilution curves, from one dog, of albumin, sucrose, and EHDP following a 47-s injection into nutrient artery (upper) with extraction derived from Eqs. 2 and 5 (lower).
Fig. 2
Fig. 2
Examples of outflow dilution curves, from one dog, of albumin, EHDP, and strontium to demonstrate greater extraction of smaller molecule strontium.
Fig. 3
Fig. 3
Example of a plasma clearance curve for EHDP over 4 h, showing rapid plasma clearance.
Fig. 4
Fig. 4
Mean percentage of injectate in various tissues of the body at 1, 2, 3, and 4 h, showing decreasing blood, increasing urine, but constant bone uptake.
Fig. 5
Fig. 5
Mean ratios of radioactivity of tissue to that of plasma at 1, 2, 3, and 4 h, from Eq. 10.
See this image and copyright information in PMC

References

    1. Bassingthwaighte JB. A concurrent flow model for extraction during transcapillary passage. Circulation Res. 1974;35:483–503. - PMC - PubMed
    1. Bauer GCH, Lindberg L, Naversten Y, Sjöstrand L-O. 85Sr radionuclide scintimetry in infected total hip arthroplasty. Acta Orthopaed. Scand. 1973;44:439–450. - PubMed
    1. Becker RR, Hassing GS, Lau JR, Saville PD. The hyperphosphatemic effect of disodium ethane-1-hydroxy-1,1-di-phosphonate (EHDF™): renal handling of phosphorus and the renal response to parathyroid hormone. J. Lab. Clin. Med. 1973;81:258–266. - PubMed
    1. Bosch WJ. Plasma 45Ca clearance by the tibia in the immature dog. Am. J. Physiol. 1969;216:1150–1157. - PubMed
    1. Bowen BM, Garnett ES. Analysis of the relationship between 99mTc-Sn-polyphosphate and 99mTc-Sn-pyrophosphate. J. Nucl. Med. 1974;15:652–655. - PubMed

Publication types

LinkOut - more resources