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. 2025 Apr 14;18(4):571.
doi: 10.3390/ph18040571.

Acute Supplementation of Soluble Mango Leaf Extract (Zynamite® S) Improves Mental Performance and Mood: A Randomized, Double-Blind, Placebo-Controlled Crossover Study

Yolanda Castellote-Caballero  1 , Ana Beltrán-Arranz  2 , Agustín Aibar-Almazán  3 , María Del Carmen Carcelén-Fraile  4 , Yulieth Rivas-Campo  5 , Laura López-Ríos  2 , Tanausú Vega-Morales  2 , Ana María González-Martín  6   7
Affiliations

Acute Supplementation of Soluble Mango Leaf Extract (Zynamite® S) Improves Mental Performance and Mood: A Randomized, Double-Blind, Placebo-Controlled Crossover Study

Yolanda Castellote-Caballero et al. Pharmaceuticals (Basel). .

Abstract

Background/Objectives: A mango (Mangifera indica) leaf extract (Zynamite®), rich in the polyphenol mangiferin, has been demonstrated to modulate brain activity, boost cognitive function, and reduce mental fatigue. Research evidence supports that improving the solubility of this extract could significantly enhance its efficacy as an active ingredient. This study examined the effects of a soluble version of Zynamite®, Zynamite® S (Zyn-S), on cognitive function and mood in young adults at low doses. Methods: A total of 119 university students were enrolled in the study. Participants were randomly assigned to receive either 100 mg, 150 mg, or placebo in a double-blind crossover design. Short- and long-term memory were evaluated using the Rey Auditory Verbal Learning Test (RAVLT), executive functions with the Trail Making Test (TMT), processing speed with the Digit Symbol Substitution Test (DSST), and selective attention with the Stroop Color and Word Test. Additionally, mood was assessed using the Spanish short version of the Profile of Mood States (POMS). All these assessments were conducted before taking the product and at 30 min, 3 h, and 5 h post-intake. Results: The results demonstrated that participants who received Zynamite® S experienced significant improvements in reduced tension, depression, and confusion, suggesting an enhancement in mental clarity and overall emotional well-being. Both interventions also improved processing speed and cognitive flexibility. However, no significant differences were observed in short- and long-term verbal memory. Conclusions: In summary, these findings support Zynamite® S as a natural nootropic capable of acutely improving key cognitive functions and emotional balance at low doses in young adults, with sustained efficacy for at least five hours.

Keywords: Mangifera indica; Zynamite; cognitive function; mood; university students.

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Conflict of interest statement

A.B.-A., L-L.-R. and T.V.-M. are employed by the company Nektium Pharma S.L., C/Las Mimosas 8, Polígono Industrial Arinaga, Las Palmas. The present investigation was financed by Nektium Pharma S.L., who provided the polyphenolic compounds and participated in the experimental design, interpretation of data, and writing of the manuscript, although they were fully excluded from participation in the experimental data collection and data analysis. The remaining authors declare that this research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

Figures

Figure 1
Figure 1
Effects of Zyn-S supplementation on (A) RAVLT Learning and (B) RAVLT Delayed Recall. Data are shown as means ± SD. Two-tailed Student’s paired t test and repeated-measures ANOVA with post hoc Bonferroni adjustment were conducted. ^ = p < 0.05 difference from baseline value.
Figure 2
Figure 2
Effects of Zyn-S supplementation on (A) TMT-A and (B) TMT-B. Data are shown as means ± SD. Two-tailed Student’s paired t test and repeated-measures ANOVA with post hoc Bonferroni adjustment were conducted. ^ = p < 0.05 difference from baseline value. * = p < 0.05 difference compared with placebo group.
Figure 3
Figure 3
Effects of Zyn-S supplementation on DSST. Data are shown as means ± SD. Two-tailed Student’s paired t test and repeated-measures ANOVA with post hoc Bonferroni adjustment were conducted. ^ = p < 0.05 difference from baseline value. * = p < 0.05 difference compared with placebo group.
Figure 4
Figure 4
Effects of Zyn-S supplementation on (A) Stroop Word and Color (B) and Stroop Interference. Data are shown as means ± SD. Two-tailed Student’s paired t test and repeated-measures ANOVA with post hoc Bonferroni adjustment were conducted. ^ = p < 0.05 difference from baseline value. * = p < 0.05 difference compared with placebo group.
Figure 5
Figure 5
Effects of Zyn-S supplementation on multiple domains of POMS: (A) Total Mood Disturbances, (B) Tension, (C) Confusion, and (D) Depression. Data are shown as means ± SD. Two-tailed Student’s paired t test and repeated-measures ANOVA with post hoc Bonferroni adjustment were conducted. ^ = p < 0.05 difference from baseline value. * = p < 0.05 difference compared with placebo group. For the rest of the emotional states evaluated (anger, fatigue, and vigor), no significant changes were observed, neither at different times compared with the baseline nor improvements between groups.
Figure 6
Figure 6
CONSORT flow diagram illustrating the flow of participants through the phases of the study and the number that withdrew at each time point.
Figure 7
Figure 7
Flow chart of the study procedures throughout the test days. POMS: Profile of Mood States. RAVLT: The Rey Auditory Verbal Learning Test. TMT: Trail Making Test. DSST: Digit Symbol Substitution Test. STROOP: Stroop Color and Word Test and Stroop Interference.
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