Green synthesis of silver and gold nanoparticles using seeds extract of Cichorium intybus and their comparative analysis with commercially available ointment for wound healing activity

Abstract

Wound healing is a complicated physiological process that involves several stages including hemostasis, inflammation, proliferation and repair to rebuild the integrity of the skin and subcutaneous tissue. Millions of people around the world are affected by poor wound healing, causing increased mortality rates and related costs. Immedicable wounds are a health problem because they directly affect the person's standard of living and produce an ultimatum to the health of people and the international economy because of the expensive medical treatment. Other schemes/approaches must be designed to achieve productive and therapeutic results. In this context, the emergence of Nano biotechnology may provide another manifesto to produce latest drugs for long-term treatment processes. This article shows that the implementation of metal nanoparticles (Au and Ag) has unbolted a brand-new gateway in the discipline of eco-friendly medicine due to their unique properties such as medicine transportation, antimicrobial activity and quick recovery. Moreover, metal nanoparticles (NPs) manufactured by green synthesis not only add the effects of nanoparticles and plant extracts but also reduce toxicity to tissues and make their use safer. In the present work, Ag and Au nanoparticles were synthesized using an aqueous seed extract of Cichorium intybus and their wound healing ability was assessed by performing in-vivo wound healing activity on albino mice. Till now there is no study available on the in-vivo wound healing potential of Ag and Au nanoparticles using Cichorium intybus. This is the first study ever to assess the ability of Cichorium intybus seed extract and its metal nanoparticles (AgNPs & AuNPs) to promote wound healing in animals which shows its uniqueness and novelty. The results displayed augmented and quicker wound closure with Ag as compared to the Au nanoparticles, polyfax and simple plant extract in a 21-day research work. These results illustrated that eco-friendly prepared nanoparticles provide a prominent approach to fight with the bacteria resistant against combination therapy without any lethal affair. Environment-friendly NPs declared to be the low-cost best treatment for the faster recovery of wounds. The main goal of this research is to provide aid in the creation of plant-based, durable and affordable Nano medicine for wound care, lowering dependency on synthetic techniques and encouraging environmental friendly biomedical applications.

Keywords: Cichorium intybus; Inflammation; Nano biotechnology; Nanoparticles; Wound.