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. 2025 Apr 26.
doi: 10.1007/s10528-025-11115-1. Online ahead of print.

IBSP Promotes Clear Cell Renal Cell Carcinoma Progression Through the PI3 K/AKT Pathway

Zhen-Hua Jin #  1 , Jun Ge #  2 , Jin-Zhuo Ning  3
Affiliations

IBSP Promotes Clear Cell Renal Cell Carcinoma Progression Through the PI3 K/AKT Pathway

Zhen-Hua Jin et al. Biochem Genet. .

Abstract

Our objective is to reveal IBSP expression within clear cell renal cell carcinoma (ccRCC) and the mechanism behind it. IBSP expression and its clinical significance in ccRCC were analyzed using the TCGA dataset. Both Western blotting and immunohistochemistry were utilized to examine IBSP expressions in clinical ccRCC specimens. Moreover, CCK-8, Annexin V-FITC/PI, wound healing, and Transwell assays were utilized to determine IBSP's role in ccRCC progression in vitro. In addition, Western blotting was deployed to ascertain levels of IBSP, PI3K/p-PI3K, and AKT/p-AKT. The TCGA database and our tissue data showcased that, unlike normal tissues, IBSP was significantly overexpressed in ccRCC tissues. Down-regulating IBSP reduces cell abilities to proliferate, migrate, and invade, besides promoting apoptosis in vitro. In vitro and in vivo, IBSP down-regulation inhibited ccRCC growth cells by suppressing PI3K/AKT phosphorylation. IBSP acts as a potential oncogene and could be a prognostic biomarker and therapeutic target for ccRCC. Furthermore, targeting IBSP may enhance existing treatment strategies, such as combining it with PI3K/AKT inhibitors to improve patient outcomes.

Keywords: CcRCC; IBSP; PI3 K/AKT signaling.

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Conflict of interest statement

Declarations. Competing Interests: The authors declare no competing interests. Ethics Approval and Consent to Participate: The study protocol was approved by the ethics committee of Renmin Hospital of Wuhan University (Permit number: WDRY2022-K051), and all aspects of the study comply with the criteria established by the Declaration of Helsinki. The tissue samples were obtained with written informed consent from each patient. Animal experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee of Wuhan University Medical College (Certificate number: WDRM20240302 A).

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