Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun:356:199579.
doi: 10.1016/j.virusres.2025.199579. Epub 2025 Apr 27.

Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp

Truong Thi Bich Van  1 , Nguyen Thi Loan Anh  2 , Van-Thanh Vo  2 , Nguyen Pham Anh Thi  2
Affiliations

Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp

Truong Thi Bich Van et al. Virus Res. 2025 Jun.

Abstract

Bacteriophages are extensively employed across various fields, including medicine, veterinary medicine, the food industry, agriculture, biotechnology, and pharmacy, owing to their numerous advantages. These advantages include rapid clearance of pathogens, self-propagation at the infection site, host specificity, potential for genetic modification, ease of isolation, stability, and low production costs. This study isolated a bacteriophage from shrimp pond wastewater in the Mekong Delta region. The bacteriophage was identified as a lytic bacteriophage belonging to the genus Bruyoghevirus, class Caudoviricetes, with the ability to effectively lyse three bacterial strains: V. parahaemolyticus, Salmonella enteritidis, and Salmonella typhimurium. Growth curve analysis revealed variations in the latency period and the number of phages produced during the life cycle across all three hosts. Bacteriophage Produced 117, 176, and 52 PFU/cell against V. parahaemolyticus, S. enteritidis, and S. typhimurium, respectively. Phage ST1749 demonstrated activity across a broad range of temperatures (-20 °C to 70 °C) and pH levels (2 to 10), with optimal stability observed at pH 5 to 7. Furthermore, phage ST1749 exhibited biofilm-degrading and lytic capabilities against the three bacterial strains studied. These findings suggest that phage ST1749 has the potential to serve as a biocontrol agent for treating infections caused by antibiotic-resistant bacteria.

Keywords: Bacteriophage st1749; Biocontrol; Biofilm; Bruyoghevirus; Caudoviricetes; Vibrio parahaemolyticus.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig. 1
Transmission electron microscopy images (A) and plaque morphology (B, C, D) of phage ST1749.
Fig 2
Fig. 2
One-step growth curve of phage ST1749.
Fig 3
Fig. 3
Thermal stability of phage ST1749.
Fig 4
Fig. 4
pH stability of phage ST1749.
Fig 5
Fig. 5
Effect of phage ST1749 on inhibition of biofilm in 96-well microplate after 24 hours. Values represent mean with a standard deviation of three determinations. * Significant at p < 0.01.
Fig 6
Fig. 6
Lytic activity of phage ST1749 at five time intervals 0, 2, 4, 6, and 24 hours. Values represent mean with a standard deviation of three determinations. * Significant at p < 0.01.
See this image and copyright information in PMC

References

    1. Abd-El Wahab A., Basiouni S., El-Seedi H.R., Ahmed M.F.E., Bielke L.R., Hargis B., Tellez-Isaias G., Eisenreich W., Lehnherr H., Kittler S., Shehata A.A., Visscher C. An overview of the use of bacteriophages in the poultry industry: successes, challenges, and possibilities for overcoming breakdowns. Front. Microbiol. 2023;14 doi: 10.3389/fmicb.2023.1136638. - DOI - PMC - PubMed
    1. Abedon S.T. In: Bacteriophages: Biology, Technology, Therapy. Harper D.R., Abedon S.T., Burrowes B.H., McConville M.L., editors. Springer International Publishing; Cham: 2021. Detection of bacteriophages: phage plaques; pp. 507–538. - DOI
    1. Abedon S.T., Yin J. In: Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions, Methods in Molecular Biology™. Clokie M.R.J., Kropinski A.M., editors. Humana Press; Totowa, NJ: 2009. Bacteriophage plaques: theory and analysis; pp. 161–174. - DOI - PubMed
    1. Alves D.R., Gaudion A., Bean J.E., Perez Esteban P., Arnot T.C., Harper D.R., Kot W., Hansen L.H., Enright M.C., Jenkins A.T.A. Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation. Appl. Environ. Microbiol. 2014;80:6694–6703. doi: 10.1128/AEM.01789-14. - DOI - PMC - PubMed
    1. Amankwah S., Abdella K., Kassa T. Bacterial biofilm destruction: a focused review on the recent use of phage-based strategies with other antibiofilm agents. Nanotechnol. Sci. Appl. 2021;14:161–177. doi: 10.2147/NSA.S325594. - DOI - PMC - PubMed

Publication types

MeSH terms

Associated data

LinkOut - more resources