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. 2025 Apr 1;26(4):1385-1391.
doi: 10.31557/APJCP.2025.26.4.1385.

Genotype and Association of XRCC1 Gene With Liver Disease in Chronic Hcv Patients

Zaid Talal Abdulqader Al-Qadhi  1 , Wahby Mohammed Ahmed Babaresh  2 , Irshad Ur Rehman  3 , Rashid Saeed Abdo Hassen  3 , Amin Allah  3 , Sameer Ahmad Ali  1 , Hasan Al-Mansoub  1 , Fouad Al-Samai  1 , Yazid Nasher Retas  2
Affiliations

Genotype and Association of XRCC1 Gene With Liver Disease in Chronic Hcv Patients

Zaid Talal Abdulqader Al-Qadhi et al. Asian Pac J Cancer Prev. .

Abstract

Chronic infections with hepatitis B virus (HBV) and hepatitis C virus (HCV) are well-established major risk factors for hepatocellular carcinoma (HCC). Nevertheless, only a minority of infected individuals progress to HCC in their lifetime, highlighting the potential influence of genetic factors in modulating susceptibility to this malignancy. The X-ray repair cross-complementing group (XRCC1) is involved in DNA repair pathways.

Aim: investigate the association between the c.24589 G>A single nucleotide polymorphism (SNP) of the XRCC1 gene and the risk of developing hepatocellular carcinoma (HCC) in patients with chronic hepatitis C virus (HCV) infection.

Methods: A case-control study was conducted in the oncology departments of Peshawar, Pakistan, involving 30 HCC patients and 30 cirrhotic HCV patients without HCC. After collecting relevant clinical data and basic laboratory tests,c.24589 G>A SNP of the XRCC1 gene was analyzed using the ARMS-PCR technique.

Results: A statistically significant higher frequency of XRCC1 (AA and GA) genotypes was observed in patients with hepatocellular carcinoma (HCC) compared to those with cirrhotic hepatitis C virus (HCV) (p = 0.0425). In addition, the A allele frequency was notably higher in the HCC group (54% compared to 23%, p = 0.0178). Moreover, multivariate analysis indicated that the c.24589 G>A SNP independently increased the risk of developing HCC by 4.58 times (95% CI: 1.7-11.88, p = 0.017). Furthermore, patients with the AA and GA genotypes displayed larger tumor sizes (p = 0.008), a greater number of tumor foci (p = 0.006), and higher Child-Pugh grades (p = 0.044).

Conclusion: XRCC1 gene polymorphism could be associated with increased risk of HCC development in chronic HCV patients.

Keywords: Chronic hepatitis C; DNA Repair; Genetic polymorphism; XRCC1 gene; liver disease.

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Conflict of interest statement

I declare no conflict of interest in relation to this research. There are no financial, professional, or personal factors that could have influenced the study’s findings.

Figures

Figure 1
Figure 1
Agarose Gel Electrophoresis Images of Extracted Samples for HCC FROM (1-25)
Figure 2
Figure 2
Agarose Gel Electrophoresis Images of Extracted Samples for HCV and Analyzed by ARMS – PCR FROM (1-25).
See this image and copyright information in PMC

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