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. 1985 Sep;5(3):465-8.
doi: 10.1038/jcbfm.1985.63.

Regional quantitative determination of brain glucose in tissue sections: a bioluminescent approach

W Paschen, G Mies, O Kloiber, K A Hossmann

Regional quantitative determination of brain glucose in tissue sections: a bioluminescent approach

W Paschen et al. J Cereb Blood Flow Metab. 1985 Sep.

Abstract

A bioluminescent technique is described for the regional quantitative determination of brain glucose. A close linear interrelationship was obtained between the optical density of the bioluminescent images and the glucose content in tissue samples. The regression coefficients of this correlation permit the quantification of glucose bioluminescent pictures using an image-processing system. Regional distribution of glucose was correlated to regional tissue pH under both physiological and pathophysiological conditions.

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