Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977;45(1-2):24-33.
doi: 10.1159/000162855.

Marked instability of character of cloned epithelial cells

H P Schnebli, M Burger, F F Strasser, E Suter

Marked instability of character of cloned epithelial cells

H P Schnebli et al. Exp Cell Biol. 1977.

Abstract

Cell lines derived from 7,12-dimethylbenzanthracene-induced rat mammary carcinomas show a marked drift in properties when cultured in vitro. The following characteristics alter progressively with the number of passages: cloning efficiency, requirement for hydrocortisone, requirement for fetal sera, growth response to conditioning factor(s) and ease of detachment from the culture vessel by trypsinization.

PubMed Disclaimer