Phytochemical composition, antioxidant potential, and insecticidal activity of Moringa oleifera extracts against Tribolium castaneum: a sustainable approach to pest management

Abstract

Moringa oleifera's rich phytochemical profile positions it as a promising natural alternative for sustainable pest management. This study characterizes the phytochemical composition and antioxidant activity of M. oleifera extracts and, for the first time, evaluates their insecticidal potential against Tribolium castaneum (red flour beetle). Extracts from leaves, stems, roots, and seeds were prepared using ethanol, methanol, acetone, and distilled water. Phytochemical analysis identified high levels of total phenolics (7.18 µg GAE/mg DW, ethanol leaf extract), flavonoids (23.52 µg QE/mg DW, ethanol leaf extract), tannins (245.4 mg CE/g DW, acetone seed extract), saponins (13.7%, methanol leaf extract), and alkaloids (30.06 mg atropine/100 mL, water stem extract). HPLC quantification revealed abundant bioactive compounds, including phenolic acids (gallic, caffeic, chlorogenic, and ferulic acids), flavonoids (apigenin, quercetin, kaempferol), flavanols (catechin, epicatechin, procyanidin dimers), and iridoids (oleuroside), with gallic acid, quercetin-3-O-rhamnoside, and kaempferol as dominant constituents. Antioxidant assays demonstrated significant activity, with methanol root extract showing 71% DPPH inhibition and methanol stem extract exhibiting the highest total antioxidant capacity (36.12 mg GAE/g DW). Insecticidal bioassays revealed ethanol root extracts induced 90% mortality in T. castaneum within 24 h at 30% concentration, outperforming other extracts. These findings underscore M. oleifera's dual functionality as a potent bioinsecticide and antioxidant, advocating for its integration into sustainable pest management. Further research should optimize extraction protocols and field-scale formulations to enhance practical applications.

Keywords: Moringa oleifera; Tribolium castaneum; Antioxidant activity; Bioactive compounds; Flavonoids; Insecticidal properties; Natural pesticides; Phytochemicals; Sustainable pest management.

Fig. 1

LC–MS chromatogram showing the separation of selected phenolics of ethanolic Moringa oleifera’s stems extract

Fig. 2

LC–MS chromatogram showing the separation of selected coumpounds of Moringa oleifera’s roots ethanolic extract

Fig. 3

LC–MS chromatogram showing the separation of selected coumponds of Moringa oleifera’s leaves extract

Fig. 4

LC–MS chromatogram showing the separation of selected coumpounds of Moringa oleifera oleifera’s leaves extract

Fig. 5

Mortality rates of T. castaneum exposed to Moringa oleifera extracts from leaves (A), seeds (B), stems (C), and roots (D)