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. 2025 Apr 25:255:215-234.
doi: 10.3897/phytokeys.255.144697. eCollection 2025.

Diving into Diversity: Hasleaberepwari (Bacillariophyceae, Naviculaceae), a new species of marine diatom from New Caledonia

Fiddy Semba Prasetiya  1 , Thierry Jauffrais  2 , Mustapha Mohamed-Benkada  3   4 , Nolwenn Callac  2 , Dominique Ansquer  2 , Elif Yılmaz  5 , Claude Lemieux  6 , Monique Turmel  6 , Jean-Luc Mouget  7 , Danang Ambar Prabowo  1 , Debora Christin Purbani  1 , Diah Radini Noerdjito  1 , Varian Fahmi  1 , Sulastri Arsad  5 , Romain Gastineau  5
Affiliations

Diving into Diversity: Hasleaberepwari (Bacillariophyceae, Naviculaceae), a new species of marine diatom from New Caledonia

Fiddy Semba Prasetiya et al. PhytoKeys. .

Abstract

The current article introduces and describes Hasleaberepwari sp. nov., a new species of diatom discovered in the vicinity of Boulouparis, New Caledonia. Under light microscopy, H.berepwari sp. nov. strongly resembles Hasleapseudostrearia, but preliminary molecular barcoding conducted using partial 18S and rbcL genes suggested that it was a distinct species. This was confirmed first by scanning electron microscopy which showed the differences in stria densities between both species. A short-reads genome-skimming protocol applied on H.berepwari sp. nov. led us to obtain its complete mitochondrial and plastid genomes. The mitogenome is 36,572 bp in length and as already observed among other species of Haslea spp., the nad6 and nad2 genes are fused within a single open-reading frame. The plastome is 131,897 bp length, and unlike the mitogenome, it is not colinear with those of H.pseudostrearia. The results derived from the sequencing of the plastome allowed to perform a 123-gene multigene maximum likelihood phylogeny that associates H.berepwari sp. nov. to H.pseudostrearia with maximum support at the nodes but also strictly distinguishes them, suggesting a greater genetic distance between these species than what has been previously observed between other marennine-producing species.

Keywords: Coral Sea; Naviculales; organellar genomes; tropical diatoms.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1.
Figure 1.
Sampling location of Hasleaberepwari in the experimental aquaculture station of Saint Vincent (21°55'36.9"S, 166°05'00.9"E), the West Coast of Grande Terre, New Caledonia, during summer 2020. Sampling point is indicated with the red dots sign. Map produced with QGIS software version 3.43.3 (QGIS.org 2023).
Figure 2.
Figure 2.
Hasleaberepwari sp. nov. in vivo pictures with two parietal chloroplasts and apices without the presence blue pigment in light microscopy (A, B) and LM image of a cleaned valve (C–E), scale bar 20µm.
Figure 3.
Figure 3.
SEM micrographs of Hasleaberepwari sp. nov. strain P05. A Whole valve in external view B whole valve in internal view C internal view of the center of the valve demonstrating accessory ribs and the presence of a thin bar on the left side of the raphe D external view of the valve centre showing approximate raphe endings and continuous longitudinal fissures E internal view of apex with the helictoglossa F external view of apex showing the curved distal raphe fissure and part of the parallel and longitudinal slits adjoining the peripheral one. Scale bars: 10 μm (A); 20 μm (B); 1 μm (C–F).
Figure 4.
Figure 4.
Mitochondrial genome of Hasleaberepwari sp. nov. Coloured boxes represent genes, with colours denoting their categories as indicated in the bottom left legend. The inner grey ring shows variations in G+C content.
Figure 5.
Figure 5.
Plastid genome of Hasleaberepwari sp. nov. Coloured boxes represent genes, with colours denoting their categories as indicated in the bottom left legend. The inner grey ring shows variations in G+C content.
Figure 6.
Figure 6.
Maximum Likelihood phylogenetic tree obtained from concatenated alignments of 123 protein coding genes from 19 species of diatoms. The tree is rooted with Eunotianaegelii.
Figure 7.
Figure 7.
Maximum Likelihood phylogenetic tree obtained from concatenated alignments of three protein coding genes from 133 species of diatoms. The tree was rooted with Triparmapacifica. The subtree containing the 14 species of Haslea spp. is shown.
See this image and copyright information in PMC

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