Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan
- PMID: 40330060
- PMCID: PMC12049747
- DOI: 10.18502/ijm.v17i1.17800
Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan
Abstract
Background and objectives: Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate Escherichia coli (E. coli) strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.
Materials and methods: Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying E. coli isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (bla CTX and bla TEM).
Results: Among 412 isolates, 198 (48.1%) were E. coli strains, followed by Staphylococcus saprophyticus, Klebsiella sp., Serratia sp., Enterococci sp. and Proteus sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs). E. coli species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of E. coli isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the E. coli isolates, 25 (12.6%) were ESBL-producers. The bla CTX-M gene was genetically confirmed in 20 (10.1%) of the isolates.
Conclusion: E. coli is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.
Keywords: Antibiotic resistance; Erythromycin; Escherichia coli; Extended-spectrum beta-lactamase; Urinary tract infections; blaCTX-M gene.
Copyright© 2025 The Author. Published by Tehran University of Medical Sciences.
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References
-
- Kaur R, Kaur R. Symptoms, risk factors, diagnosis and treatment of urinary tract infections. Postgrad Med J 2021; 97: 803–812. - PubMed
-
- Sahu R, Sahoo RK, Prusty SK, Sahu PK. Urinary tract infection and its management. Syst Rev Pharm 2019; 10: 42–48.
-
- Salari N, Khoshbakht Y, Hemmati M, Khodayari Y, Khaleghi AA, Jafari F, et al. Global prevalence of urinary tract infection in pregnant mothers: a systematic review and meta-analysis. Public Health 2023; 224: 58–65. - PubMed
-
- Víquez-Molina G, Aragón-Sánchez J, Pérez-Corrales C, Murillo-Vargas C, López-Valverde ME, Lipsky BA. Virulence factor genes in Staphylococcus aureus isolated from Diabetic foot soft tissue and bone infections. Int J Low Extrem Wounds 2018; 17: 36–41. - PubMed
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