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. 2025 Apr 21;30(8):1855.
doi: 10.3390/molecules30081855.

Deep Eutectic Solvents (DESs) as Alternative Sustainable Media for the Extraction and Characterization of Bioactive Compounds from Winemaking Industry Wastes

Vincenzo Roselli  1   2 , Rosalba Leuci  1 , Gianluca Pugliese  3 , Alexia Barbarossa  1 , Antonio Laghezza  1 , Marco Paparella  1 , Alessia Carocci  1 , Vincenzo Tufarelli  3 , Lucia Gambacorta  2 , Luca Piemontese  1
Affiliations

Deep Eutectic Solvents (DESs) as Alternative Sustainable Media for the Extraction and Characterization of Bioactive Compounds from Winemaking Industry Wastes

Vincenzo Roselli et al. Molecules. .

Abstract

The increasing pollution and wastage of food and byproducts from agro-industrial production is an increasingly worrying issue. Grape is one of the most diffused fruit crops cultivated, and grape pomace is the main solid byproduct obtained in the winemaking process; interestingly, it is rich in health-beneficial bioactive molecules. In order to recover these molecules, in this work, a green method has been developed, considering two grape pomaces from different cultivars, namely, Petit Verdot and Cabernet Sauvignon. The extraction procedure, as the first step of this process, was carried out with seven selected deep eutectic solvents (DESs). Then, analysis using HPLC-DAD allowed the detection and quantification of eight out of fifteen different phenolic compounds under examination in the extracts produced, including three quercetin glucosides. The evaluation of antioxidant activity, through the DPPH photometric assay, led to the selection of choline chloride/urea 1:2 + 40% water DES extracts as the extracts with the most promising results. Moreover, significant antibacterial activity was also achieved, in particular, for the betaine/lactic acid 1:4 + 40% water DES extract. Further studies will employ this method for numerous cultivars of grape pomaces with the ambitious aim of the production of polyphenol-enriched food and feed supplements.

Keywords: antimicrobials; antioxidants; deep eutectic solvents (DESs); green chemistry; green extraction methods; winemaking industry wastes.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Sum of levels of the eight polyphenols in fresh and dried Cabernet Sauvignon and in fresh and dried Petit Verdot grape pomace according HPLC-DAD analysis; all the DESs included +40% water.
Figure 2
Figure 2
Antioxidant activity calculated, for each extract, as gallic acid equivalents (GAEs) through DPPH photometric assay. FC: fresh Cabernet Sauvignon; DC: dry Cabernet Sauvignon; FP: fresh Petit Verdot; DP: dry Petit Verdot.
See this image and copyright information in PMC

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