Sex-biased fertility degeneration induced by depletion of an auxiliary piRNA-pathway factor Qin in Bombyx mori

Abstract

The PIWI-interacting RNA (piRNA) pathway is the major defense system for transposable elements (TEs) silencing in animal gonads, maintaining genomic integrity of germ cells and ensuring proper gametogenesis. An the piRNA-pathway factor, Qin, has been reported to participate in piRNA biogenesis in the lepidopteran model insect, Bombyx mori. Nevertheless, the physiological functions of Qin remain to be characterized. Here we demonstrated that Qin plays important roles in silkworm gonad development of both sexes. BmQin was predominantly expressed in gonads. Immunofluorescent staining revealed that BmQin is localized in the cytoplasm of both germ cells and somatic cells in gonads. Depletion of BmQin via CRISPR/Cas9 system induceed complete sterile in males, and partial sterile in females. Notably, mutants displayed severe defects in gonad development and gametogenesis. RNA-seq analysis revealed that the piRNA pathway was dysregulated in mutant gonads. In addition, apoptosis was significantly enhanced in mutant gonads. Our study revealed the physiological functions of BmQin in silkworm fertility and its auxiliary roles in the piRNA pathway in both male and female gonads.

Keywords: Bombyx mori; Fertility; Gametogenesis; Qin; piRNA pathway.