doi: 10.1038/316601a0.
Changing the binding specificity of a repressor by redesigning an alpha-helix
- PMID: 4033758
- DOI: 10.1038/316601a0
Item in Clipboard
Changing the binding specificity of a repressor by redesigning an alpha-helix
Nature.
1985 Aug.
Abstract
We replaced amino acids on the 'outside', or solvent-exposed, surface of the DNA recognition alpha-helix of 434 repressor with the corresponding amino acids from the recognition helix of P22 repressor. The binding specificity of the resulting hybrid protein, as measured in vivo and in vitro, was that of P22 repressor.
Similar articles
-
A phage repressor-operator complex at 7 A resolution.Nature. 1985 Aug 15-21;316(6029):596-601. doi: 10.1038/316596a0. Nature. 1985. PMID: 4033757
-
Isolation and analysis of arc repressor mutants: evidence for an unusual mechanism of DNA binding.Proteins. 1986 Dec;1(4):302-11. doi: 10.1002/prot.340010404. Proteins. 1986. PMID: 3449859
-
Recognition of DNA sequences by the repressor of bacteriophage 434.Biophys Chem. 1988 Feb;29(1-2):31-7. doi: 10.1016/0301-4622(88)87022-4. Biophys Chem. 1988. PMID: 3359001
-
Protein-DNA recognition.Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453. Annu Rev Biochem. 1984. PMID: 6236744 Review.
-
[DNA-recognition by helix-turn-helix variants in DNA-binding domains].Tanpakushitsu Kakusan Koso. 2000 Jun;45(9 Suppl):1683-93. Tanpakushitsu Kakusan Koso. 2000. PMID: 10879150 Review. Japanese. No abstract available.
Cited by
-
A permutational approach toward protein-DNA recognition.Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3969-73. doi: 10.1073/pnas.91.9.3969. Proc Natl Acad Sci U S A. 1994. PMID: 8171021 Free PMC article.
-
Cloning and characterization of the MboII restriction-modification system.Nucleic Acids Res. 1991 Mar 11;19(5):1007-13. doi: 10.1093/nar/19.5.1007. Nucleic Acids Res. 1991. PMID: 2020540 Free PMC article.
-
The 43 residue DNA binding domain of gamma delta resolvase binds adjacent major and minor grooves of DNA.Nucleic Acids Res. 1989 Feb 11;17(3):1035-50. doi: 10.1093/nar/17.3.1035. Nucleic Acids Res. 1989. PMID: 2537948 Free PMC article.
-
Tn10 tet operator mutations affecting Tet repressor recognition.Nucleic Acids Res. 1986 May 27;14(10):4253-66. doi: 10.1093/nar/14.10.4253. Nucleic Acids Res. 1986. PMID: 3086838 Free PMC article.
-
Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter.Nucleic Acids Res. 1987 Dec 10;15(23):9945-56. doi: 10.1093/nar/15.23.9945. Nucleic Acids Res. 1987. PMID: 3320958 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
