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. 2025 May 8;207(6):137.
doi: 10.1007/s00203-025-04344-w.

Genome-wide identification of CYP450 in Ganoderma lucidum and expression analysis of genes related to ganoderic acid synthesis

Xiaolu Li  1   2 , Dong Liu  2 , Bangxing Han  2 , Shiping Huang  3 , Hui Deng  4
Affiliations

Genome-wide identification of CYP450 in Ganoderma lucidum and expression analysis of genes related to ganoderic acid synthesis

Xiaolu Li et al. Arch Microbiol. .

Abstract

The biosynthesis mechanism of ganoderic acid, a class of secondary metabolites of Ganoderma lucidum, is currently unknown. Members of G. lucidum's CYP450 gene family were found in this study by genome-wide analysis. Determination of ganoderic acid content in Jilin, Zhejiang, Jinzhai, Fujian, Yunnan and Shandong regions was performed using high performance liquid chromatography. Expression of CYP450 gene of G. lucidum from Jilin, Jinzhai and Shandong origins was analyzed by transcriptome sequencing. The findings indicated that, through comprehensive sequence analysis and functional annotation, 189 CYP450 genes were detected in the G. lucidum genome, among which 34 CYP450 genes were significantly differentially expressed in transcriptome analysis, and the gene expression was positively correlated with ganoderic acid content. Bioinformatics analysis predicted the conserved motifs, structural features, and subcellular localization of 189 CYP genes, revealing significant differences in gene structure and protein motif composition among GlCYP450 family members. Subcellular localization revealed that they are located in the plasma membrane, cytoplasm, nucleus, mitochondria, peroxisome, endoplasmic reticulum, extracellular space, and cytoskeleton, suggesting that they have multiple functions. A number of cis-regulatory elements associated with stress responses and phytohormones were identified in the promoter regions of these genes. It demonstrates that ganoderic acid production has been significantly controlled by these P450 genes. These findings offer a crucial theoretical foundation for a thorough comprehension of the process of ganoderic acid production, the bioinformatics role of CYP450 genes in G. lucidum, and the selection and breeding of superior G. lucidum resources.

Keywords: CYP450; Ganoderma lucidum; Ganoderic acid; Genome-wide identification; Origin differences; Transcriptome sequencing.

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Conflict of interest statement

Declarations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.

References

    1. Ahmad R et al (2021) Ganoderma lucidum (Reishi) an edible mushroom; a comprehensive and critical review of its nutritional, cosmeceutical, mycochemical, Pharmacological, clinical, and toxicological properties. Phytother Res 35:6030–6062. https://doi.org/10.1002/ptr.7215
    1. Barbieri A et al (2017) Anticancer and Anti-Inflammatory properties of ganoderma lucidum extract effects on melanoma and Triple-Negative breast Cancer treatment. Nutrients 9. https://doi.org/10.3390/nu9030210
    1. Chen S et al (2012) Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nat Commun 3:913. https://doi.org/10.1038/ncomms1923
    1. Chen S et al (2017) Cytotoxic lanostane-type triterpenoids from the fruiting bodies of Ganoderma lucidum and their structure-activity relationships. Oncotarget 8:10071–10084. https://doi.org/10.18632/oncotarget.14336 - DOI - PubMed
    1. Chen C et al (2020) TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant 13:1194–1202. https://doi.org/10.1016/j.molp.2020.06.009 - DOI - PubMed

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