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. 2025 May 8;12(1):762.
doi: 10.1038/s41597-025-05073-x.

Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1

Le Wang  1 , Ruifang Jia  1 , Jianjun Hao  2 , Zhengqiang Chen  1 , Xiaoluo Zhang  3 , Na Wang  1 , Shengze Wang  1 , Yuanyuan Zhang  4 , Kejian Lin  5
Affiliations

Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1

Le Wang et al. Sci Data. .

Abstract

Fusarium neocosmosporiellum causes alfalfa root rot, leading to significant economic losses. To explore the genetics of F. neocosmosporiellum, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 was assembled using both Nanopore long-read and Illumina short-read sequencing technologies. The assembled genome measured 63,424,297 base pairs (bp) in size, with a contig N50 of 6,480,858 bp and a contig N90 of 3,230,245 bp. A total of 10,486,459 bp repeat sequences and 28,006 protein-coding genes were predicted, of which 15,389 were functionally annotated. This study enhances our understanding of the genetic basis for F. neocosmosporiellum's pathogenicity, providing a foundation for future studies on disease control and fungal evolution.

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Conflict of interest statement

Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Alfalfa (Medicago sativa L.) root rot disease and morphology of the fungal strain CA18-1. (A) Plant wilting. (B) Browning and decaying tissue at the plant stem base. (C) Top view of the colony cultured on potato dextrose agar (PDA) plates after seven days of incubation. (D) Microconidia. (E) Ascospores with ornate walls. Scale bars are 10 μm (D) and 20 μm (E).
Fig. 2
Fig. 2
Circular map of Fusarium neocosmosporiellum strain CA18-1 genome assembly. (a) Physical locations of 17 contigs. Bar = 1 kb. (b,c) Protein-coding genes on the forward and reverse strands, annotated with KOG classes. (d) Gene density. (e) Transposable element (TE) density. (f) Tanden repeat density.
Fig. 3
Fig. 3
Length distribution of predicted protein-coding genes in Fusarium neocosmosporiellum strain CA18-1.
Fig. 4
Fig. 4
k-mer (k = 17) frequency distribution generated using Illumina sequencing data of Fusarium neocosmosporiellum strain CA18-1. The x-axis shows k-mer depth (coverage), while the y-axis represents frequency. The primary peak at ~40X indicates the dominant homozygous genome component, with the minor peak at ~20X representing heterozygous regions (heterozygosity rate: 0.0807%).
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