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. 2025 Mar 10;12(7):uhaf081.
doi: 10.1093/hr/uhaf081. eCollection 2025 Jul.

Advances in basic biology of alfalfa (Medicago sativa L.): a comprehensive overview

Yuanyuan Zhang  1   2   3 , Lei Wang  1   2   3   4   5
Affiliations

Advances in basic biology of alfalfa (Medicago sativa L.): a comprehensive overview

Yuanyuan Zhang et al. Hortic Res. .

Abstract

Alfalfa (Medicago sativa L.), a perennial legume forage, has been broadly cultivated owing to a variety of favorable characteristics, including comprehensive ecological adaptability, superior nutritive value and palatability, and nitrogen fixation capacity. The productivity traits of alfalfa, specifically its biomass yield and forage quality, are significantly influenced by a series of determinants, including internal developmental factors and external environmental cues. However, the regulatory mechanisms underlying the fundamental biological problems of alfalfa remain elusive. Here, we conducted a comprehensive review focusing on the genomics of alfalfa, advancements in gene-editing technologies, and the identification of genes that control pivotal agronomic characteristics, including biomass formation, nutritional quality, flowering time, and resistance to various stresses. Moreover, a molecular design roadmap for the 'ideal alfalfa' has been proposed and the potential of pangenomes, self-incompatibility mechanisms, de novo domestication, and intelligent breeding strategies to enhance alfalfa's yield, quality, and resilience were further discussed. This review will provide comprehensive information on the basic biology of alfalfa and offer new insights for the cultivation of ideal alfalfa.

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Conflict of interest statement

All authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Alfalfa development stages. The growth and development stages of alfalfa include: seed germination, sprouting, seedling (or resprouting) stage, branching stage, budding stage, mowing, and postmowing regrowth stage.
Figure 2
Figure 2
Agronomic traits and distinctive characteristics affecting the yield and quality of alfalfa.
Figure 3
Figure 3
The gene regulatory network for critical agronomic traits in alfalfa.
Figure 4
Figure 4
Molecular design roadmap for ‘ideal alfalfa’.
See this image and copyright information in PMC

References

    1. Chen H, Zeng Y, Yang Y. et al. Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa. Nat Commun. 2020;11:2494. - PMC - PubMed
    1. Zhou C, Han L, Pislariu C. et al. From model to crop: functional analysis of a STAY-GREEN gene in the model legume Medicago truncatula and effective use of the gene for alfalfa improvement. Plant Physiol. 2011;157:1483–96 - PMC - PubMed
    1. Singer SD, Hannoufa A, Acharya S. Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment. Plant Cell Environ. 2018;41:1955–71 - PubMed
    1. Lorenzo CD, García-Gagliardi P, Antonietti MS. et al. Improvement of alfalfa forage quality and management through the down-regulation of MsFTa1. Plant Biotechnol J. 2020;18:944–54 - PMC - PubMed
    1. Latif A, Sun Y, Noman A. Herbaceous alfalfa plant as a multipurpose crop and predominant forage specie in Pakistan. Front Sustain Food Syst. 2023;7:1126151

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