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. 2025:2917:87-97.
doi: 10.1007/978-1-0716-4478-2_8.

Gel Zymography for the Evaluation of Matrix Metalloproteinase-2 and -9 in Aorta and Kidney Tissues

Grazieli Beatriz de Oliveira Gonçalves  1 , Vanessa de Almeida Belo  2 , Carla Speroni Ceron  3
Affiliations

Gel Zymography for the Evaluation of Matrix Metalloproteinase-2 and -9 in Aorta and Kidney Tissues

Grazieli Beatriz de Oliveira Gonçalves et al. Methods Mol Biol. 2025.

Abstract

Matrix metalloproteinases (MMPs) are endopeptidases containing zinc that play a role in extracellular matrix remodeling processes in cardiovascular or kidney diseases. The levels and activity of MMP-2 and MMP-9 increase in experimental models of diabetic nephropathy, acute kidney injury (AKI), and renal tubular injury. MMP-2 is also induced in human renal transplants with delayed graft function (DGF) and is correlated with tubular damage in patients with tubular cell necrosis. Moreover, MMP-2 and -9 participate in the degradation of structural components of the extracellular matrix, mainly collagen and elastin, leading to destabilization and dilatation of the aorta in aneurysms. Zymography is a simple, sensitive, quantitative, and widely used approach to assessing both latent and active forms of MMPs in biological materials.

Keywords: Aorta; Gelatin zymography; Gelatinases; Kidney.

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References

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