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. 2025:2917:143-150.
doi: 10.1007/978-1-0716-4478-2_13.

Uncovering Hyaluronidase Activity Using Hyaluronan Zymography in Biological Samples

Kristina Nešporová  1 , Kateřina Lehká  2 , Pavlína Musilová  2   3
Affiliations

Uncovering Hyaluronidase Activity Using Hyaluronan Zymography in Biological Samples

Kristina Nešporová et al. Methods Mol Biol. 2025.

Abstract

Hyaluronan and hyaluronidases are critical in tissue remodeling, inflammation, and tumor progression. This chapter provides a comprehensive guide to hyaluronan zymography, a powerful technique for detecting and quantifying hyaluronidase activity in complex biological samples. The method involves separating proteins by polyacrylamide gel electrophoresis with hyaluronan incorporated into the gel matrix. Following electrophoresis, the gel is incubated to allow hyaluronidases to degrade the hyaluronan substrate, resulting in clear digestion zones. Detailed protocols for sample preparation and the zymographic process are included, offering researchers a robust tool for studying hyaluronidase activity and regulation in various biological contexts.

Keywords: Enzyme pH optima; Extracellular matrix degradation; Hyaluronan zymography; Hyaluronidase activity; Skin cells.

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References

    1. McAtee CO, Barycki JJ, Simpson MA (2014) Emerging roles for hyaluronidase in cancer metastasis and therapy. Adv Cancer Res 123:1–34 - DOI - PubMed - PMC
    1. Aya KL, Stern R (2014) Hyaluronan in wound healing. Wound Repair Regen 22:579–593 - DOI - PubMed
    1. Žádníková P, Šínová R, Pavlík V et al (2022) The degradation of Hyaluronan in the skin. Biomol Ther 12(2):251
    1. Starr CR, Engleberg NC (2006) Role of hyaluronidase in subcutaneous spread and growth of group a streptococcus. Infect Immun 74(1):40–48 - DOI - PubMed
    1. Isoyama T, Thwaites D, Selzer MG et al (2005) Differential selectivity of hyaluronidase inhibitors toward acidic and basic hyaluronidases. Glycobiology 16(1):11–21 - DOI - PubMed

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