Reliable sensory processing of superficial cortical interneurons is modulated by behavioral state

Abstract

GABAergic interneurons in cortical layer 1 (L1) integrate sensory and top-down inputs to modulate network activity and support learning-related plasticity. However, little is known about how sensory inputs drive L1 interneuron activity. We used two-photon calcium imaging to measure sound-evoked responses in two L1 interneuron populations expressing vasoactive intestinal peptide (VIP) or neuron-derived neurotrophic factor (NDNF) in mouse auditory cortex. We found that L1 interneurons respond to both simple and complex sounds, but their responses are highly variable across trials. Despite this variability, these interneurons respond reliably to a narrow range of stimuli, reflecting selectivity for specific spectrotemporal sound features. Response reliability was modulated by behavioral state and predicted by the activity of neighboring interneurons. These findings reveal that L1 interneurons exhibit sensory tuning and identify the modulation of response reliability as a potential mechanism by which L1 relays state-dependent cues to shape sensory representations.

Keywords: CP: Neuroscience; GABAergic inhibitory interneurons; auditory cortex; frequency tuning; layer 1; locomotion; receptive field; reliability; stimulus coding; two-photon calcium imaging.