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. 2025 Jun;522(1):279-284.
doi: 10.1134/S1607672925600149. Epub 2025 May 11.

New Cellular Partners of HIV-1 Integrase and their Role in Viral Replication

Y Y Agapkina  1   2 , T Y Ponomareva  3 , M V Vdovina  4 , R H Ziganshin  5 , A A Rozina  3 , A N Anisenko  4   3 , M B Gottikh  4   3
Affiliations

New Cellular Partners of HIV-1 Integrase and their Role in Viral Replication

Y Y Agapkina et al. Dokl Biochem Biophys. 2025 Jun.

Abstract

Cellular proteins, partners of viral enzymes, are involved in the replication of human immunodeficiency virus type I (HIV-1) at various stages. Thus, the viral enzyme integrase, participating in several stages of the viral cycle, interacts with various cellular proteins. A number of them are already known, and for some the mechanism of action has been established, but the search for cellular partners of integrase continues. In this work, the identification of cellular partners of HIV-1 integrase has been carried out by the method of cross-linking followed by mass spectrometry (XL-MS). Twelve new potential integrase partners have been identified, and some of them have been examined for their effect on the early stages of HIV-1 replication.

Keywords: HIV-1; cellular partners of integrase; cross-linking followed by mass spectrometry; integrase.

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Conflict of interest statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: This work does not contain any studies involving human and animal subjects. CONFLICT OF INTEREST: The authors of this work declare that they have no conflicts of interest.

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