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. 2025 May 7:18:6037-6047.
doi: 10.2147/JIR.S507468. eCollection 2025.

Exploring the Mechanism of Action of Xuebijing Injection in Treating Acute Respiratory Distress Syndrome Based on Network Pharmacology and Animal Experiments

Jin Li #  1 , Meirong Shen #  2 , Zuan Yin  3
Affiliations

Exploring the Mechanism of Action of Xuebijing Injection in Treating Acute Respiratory Distress Syndrome Based on Network Pharmacology and Animal Experiments

Jin Li et al. J Inflamm Res. .

Abstract

Objective: To explore the mechanism of Xuebijing injection (XBJ) in treating acute respiratory distress syndrome (ARDS) using network pharmacology and animal experiments.

Methods: Active ingredients of XBJ were analyzed via TCMSP, and ARDS-related targets were identified through DisGENET and Genecard. Intersection targets were obtained using PubChem and Venn diagrams. Protein interaction networks, GO, and KEGG enrichment analyses were conducted. An ARDS rat model was established using lipopolysaccharide (LPS), and rats were divided into control, LPS, and XBJ-treated groups (low, medium, high doses, n=10). Lung wet/dry (W/D) ratio, inflammatory cytokines (IL-17, IL-6, IL-1β, TNF-α), MPO levels, lung pathology, and protein expression of ICAM-1 and HIF-1α were assessed via ELISA, HE staining, immunohistochemistry, and Western blot.

Results: A total of 204 ARDS targets were identified, with 46 intersection targets, mainly TNF-α, MPO, HIF-1α, and ICAM-1. XBJ affected ARDS through IL-17, HIF-1, and TNF signaling pathways. In vivo, LPS-induced lung injury showed alveolar destruction, edema, and inflammation, with increased W/D ratio, cytokines, MPO, and protein expression of HIF-1α and ICAM-1 (P<0.05). XBJ treatment alleviated lung damage, reduced inflammation, and improved pathology in a dose-dependent manner (P<0.05).

Conclusion: XBJ alleviates ARDS by regulating immune function, oxidative stress, and inflammation through IL-17, HIF-1, and TNF pathways.

Keywords: Xuebijing injection; acute respiratory distress syndrome; lipopolysaccharide; network pharmacology.

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Conflict of interest statement

All authors declare no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Venn Diagram of XBJ and ARDS related targets.
Figure 2
Figure 2
Protein interaction network diagram of 46 intersection targets.
Figure 3
Figure 3
Plots of 24 core targets with a Degree≥30.
Figure 4
Figure 4
GO enrichment analysis diagram.
Figure 5
Figure 5
KEGG enrichment analysis diagram.
Figure 6
Figure 6
Lung histopathological changes (HE, ×200).
Figure 7
Figure 7
Expressions of HIF-1α and ICAM-1 proteins in lung tissue (Western blot).
See this image and copyright information in PMC

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References

    1. Dupuis J, Sirois MG, Rhéaume E. et al. Colchicine reduces lung injury in experimental acute respiratory distress syndrome. PLoS One. 2020;15(12):e0242318. doi:10.1371/journal.pone.0242318 - DOI - PMC - PubMed
    1. Wang X, Liu F, Xu M, Wu L. Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute respiratory distress syndrome in cells via regulating autophagy-related pathway. Mol Med Rep. 2021;23(2):1. - PMC - PubMed
    1. Meyer NJ, Gattinoni L, Calfee CS. Acute respiratory distress syndrome. Lancet. 2021;398(10300):622–637. doi:10.1016/S0140-6736(21)00439-6 - DOI - PMC - PubMed
    1. Li C, Wang P, Zhang L, et al. Efficacy and safety of Xuebijing injection (a Chinese patent) for sepsis: a meta-analysis of randomized controlled trials. J Ethnopharmacol. 2018;224:512–521. doi:10.1016/j.jep.2018.05.043 - DOI - PubMed
    1. Dong R, Huang R, Shi X, Xu Z, Mang J. Exploration of the mechanism of luteolin against ischemic stroke based on network pharmacology, molecular docking and experimental verification. Bioengineered. 2021;12(2):12274–12293. doi:10.1080/21655979.2021.2006966 - DOI - PMC - PubMed

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