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. 1977 Apr 8;196(4286):202-5.
doi: 10.1126/science.403604.

Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis

M I Lomax, R B Helling, L I Hecker, S D Schwartzbach, W E Barnett

Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis

M I Lomax et al. Science. .

Abstract

Fragments of Euglena chloroplast DNA generated by endonuclease R-Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA, Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.

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