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. 2025 Jan 13;34(2):83-91.
doi: 10.1055/a-2504-1911. eCollection 2025 Jun.

MicroRNA Profiling in Chronic Limb-Threatening Ischemia Their Role in Arteriogenesis

Muhamad T Ismail  1 , Budi Y Setianto  1 , Sofia M Haryana  2 , Dyah W Anggrahini  1
Affiliations

MicroRNA Profiling in Chronic Limb-Threatening Ischemia Their Role in Arteriogenesis

Muhamad T Ismail et al. Int J Angiol. .

Abstract

Chronic limb-threatening ischemia (CLTI) represents poses a substantial threat with escalating mortality and amputation rates. Despite the existence of various clinical techniques for diagnosing CLTI, the role of microRNAs (miRNAs) in arteriogenesis remains ambiguous. Comprehensive knowledge on miRNAs may facilitate the advancement of targeted therapy pertaining to the enhancement of collateral blood flow in obstructed vessels. Therefore, this study aimed at analyzing arteriogenesis-associated plasma miRNA profiles in patients with CLTI using gene expression. Samples were acquired from the collateral arteries (CA group, n = 3) and the contralateral healthy limb (healthy artery; HA group, n = 3) of a single set of patients with CLTI. The RNA extracted from the samples was assessed for concentration and purity. A normalization factor was used to address variations in analyte abundance and/or quality across the samples. Subsequently, individual RNA molecules were directly quantified and subjected to comparative analysis between the CA and HA groups to identify the miRNAs involved in arteriogenesis. The five arteriogenesis-related miRNAs exhibiting maximum upregulation were miR-301b-3p, miR-221-5p, miR-639, miR-34a-5p, and let-7a-5p, while the five most downregulated miRNAs included miR-151a-5p, miR-371a-5p, miR-651-5p, miR-510-5p, and miR-660-5p. Summarily, this study documented marked upregulation and downregulation of miRNAs associated with arteriogenesis in the collateral arteries of patients with CLTI as compared with their contralateral healthy limbs. Possible mechanisms involved, including the regulation of YAP/TAZ pathway, TGFBR3 mRNA, SIRT1 expression, and other processes have shown to be modulated by miRNAs fluctuations.

Keywords: SIRT1 expression; TGFBR3 mRNA; YAP/TAZ pathway; arteriogenesis; chronic limb-threatening ischemia; microRNA; sequencing.

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Conflict of interest statement

Conflict of Interest None declared.

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References

    1. Diehm C, Lange S, Darius H et al.Association of low ankle brachial index with high mortality in primary care. Eur Heart J. 2006;27(14):1743–1749. - PubMed
    1. ESC Scientific Document Group . Aboyans V, Ricco J-B, Bartelink M EL et al.ESC guidelines on the diagnosis and treatment of peripheral arterial diseases, in collaboration with the European Society for Vascular Surgery (ESVS) Eur Heart J. 2018;39(09):763–816. - PubMed
    1. Berger J S, Hochman J, Lobach I, Adelman M A, Riles T S, Rockman C B. Modifiable risk factor burden and the prevalence of peripheral artery disease in different vascular territories. J Vasc Surg. 2013;58(03):673–810. - PubMed
    1. Varu V N, Hogg M E, Kibbe M R. Critical limb ischemia. J Vasc Surg. 2010;51(01):230–241. - PubMed
    1. TASC II Working Group Norgren L, Hiatt W R, Dormandy J A, Nehler M R, Harris K A, Fowkes F G.Inter-society consensus for the management of peripheral arterial disease (TASC II) J Vasc Surg 200745(1, suppl S):S5–S67. - PubMed