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. 2025:2916:139-151.
doi: 10.1007/978-1-0716-4470-6_13.

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission

Karelia Velázquez  1 , Mari Carmen Vives  2 , Susana Ruiz-Ruiz  1 , José Guerri  1 , Daniel Ventimilla  3 , Javier Terol  3 , Miguel A Pérez-Amador  4 , Manuel Talón  3 , Francisco R Tadeo  5
Affiliations

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission

Karelia Velázquez et al. Methods Mol Biol. 2025.

Abstract

Virus-induced gene silencing (VIGS) is a powerful and cost-effective biotechnological tool for studying gene function in plants. It allows for rapid downregulation of gene expression without the need for tissue culture or genetic transformation. While VIGS is transient and may pose challenges for certain developmental genes, it has been successfully used to study organ abscission. Using a VIGS protocol based on Citrus leaf blotch virus (CLBV) viral vectors, we were able to characterize the regulatory role of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-like genes and their potential HAESA (HAE)-like receptors in the process of corolla abscission in Nicotiana benthamiana flowers.

Keywords: Citrus leaf blotch virus (CLBV); Corolla abscission; Gene function; Nicotiana benthamiana; Virus-induced gene silencing (VIGS).

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