Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2916:139-151.
doi: 10.1007/978-1-0716-4470-6_13.

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission

Karelia Velázquez  1 , Mari Carmen Vives  2 , Susana Ruiz-Ruiz  1 , José Guerri  1 , Daniel Ventimilla  3 , Javier Terol  3 , Miguel A Pérez-Amador  4 , Manuel Talón  3 , Francisco R Tadeo  5
Affiliations

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission

Karelia Velázquez et al. Methods Mol Biol. 2025.

Abstract

Virus-induced gene silencing (VIGS) is a powerful and cost-effective biotechnological tool for studying gene function in plants. It allows for rapid downregulation of gene expression without the need for tissue culture or genetic transformation. While VIGS is transient and may pose challenges for certain developmental genes, it has been successfully used to study organ abscission. Using a VIGS protocol based on Citrus leaf blotch virus (CLBV) viral vectors, we were able to characterize the regulatory role of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-like genes and their potential HAESA (HAE)-like receptors in the process of corolla abscission in Nicotiana benthamiana flowers.

Keywords: Citrus leaf blotch virus (CLBV); Corolla abscission; Gene function; Nicotiana benthamiana; Virus-induced gene silencing (VIGS).

PubMed Disclaimer

References

    1. von Mohl H (1860) Ueber die anatomischen Veränderungen des Blattgelenkes, welche das Abfallen der Blätter herbeiführen. Bot Zeitg 18:1–7
    1. Tranbarger TJ, Tadeo FR (2020) Diversity and functional dynamics of fleshy fruit abscission zones. Annu Plant Rev 3:151–214
    1. Jiang C-Z, Lu F, Imsabai W, Meir S, Reid MS (2008) Silencing polygalacturonase expression inhibits tomato petiole abscission. J Exp Bot 59:973–979 - DOI - PubMed
    1. Hu F, Dong J, Zhang S, Song Z, Guan W, Yuan F, Zhong J, Liu J, Hu K, Cheng J (2023) Fine mapping and gene silencing pinpoint Capana10g002229 as a strong candidate gene regulating the deciduous character of ripe pepper fruit (capsicum spp.). Theor Appl Genet 136:107 - DOI - PubMed
    1. Estornell LH, Agustí J, Merelo P, Talón M, Tadeo FR (2013) Elucidating mechanisms underlying organ abscission. Plant Sci 199–200:48–60 - DOI - PubMed

LinkOut - more resources