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. 2025 May 15;25(1):386.
doi: 10.1186/s12887-025-05710-9.

Association of STAT6 gene polymorphism with atopic asthma among Yemeni children in Sana'a city, Yemen

Haitham Abdulwahab Masood  1 , Arwa Mohammed Othman  2 , Najla Nasr Addin Al-Sonboli  3 , Faiza Abdulnoor Ghlab  2 , Naif Mohammed Al-Haidary  2 , Muhanna M Al-Shaibani  4
Affiliations

Association of STAT6 gene polymorphism with atopic asthma among Yemeni children in Sana'a city, Yemen

Haitham Abdulwahab Masood et al. BMC Pediatr. .

Abstract

Background: Asthma is the most common chronic illness in children and is characterized by airway hyperresponsiveness, increased production of mucus, and significant inflammation. The signal transducer and activator of transcription 6 (STAT6) is a crucial gene in immune response, specifically in atopic reactions. It plays a role in the IL-4 and IL-13 signaling pathways in asthma and allergies.

Objective: This study aimed to determine the association between the STAT6 rs324011 gene polymorphism and atopic asthma among Yemeni children as well as to investigate the impact of the STAT6 rs324011 polymorphism on IL-13, total IgE, and eosinophils.

Methods: This study included 75 Yemeni children diagnosed with bronchial asthma and 75 healthy controls matched for age and sex. The STAT6 polymorphism (rs324011) was genotyped using RFLP PCR, the IL-13 serum level was measured via ELISA, and the serum IgE level was measured via electrochemiluminescence.

Results: Under a recessive model, the TT genotype of the STAT6 polymorphism rs324011 was significantly associated with an increased risk of atopic asthma compared to the CC and CT genotypes (χ2 = 6.6, OR = 2.5, CI = 1.2-5, p = 0.01). IgE levels among asthmatic children were significantly elevated in individuals with the TT genotype compared with those with the CC or CT genotypes (p = 0.04).

Conclusion: The TT genotype and T allele of the STAT6 rs324011 polymorphism may be associated with increased susceptibility to pediatric asthma among Yemeni children.

Keywords: Atopic asthma; Eosinophils; IL-13; IgE; rs324011 SNP.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: The study protocol was approved by Ethical Research Committee at Faculty of Medicine and Health Sciences, Sana’a University. In accordance with ethical guidelines, written informed consent was obtained from the parents/legal guardians of all participants under 16 years of age prior to their inclusion in the study. The study’s procedures, risks, and benefits were explained to the guardians, and their written consent was secured before involving the minors in the research. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Conflict of interest: The authors report no conflicts of interest. Clinical trial number: Clinical trial number: not applicable.

Figures

Fig. 1
Fig. 1
Genotype results. Illustrates the genotyping results for the STAT6 rs324011 polymorphism using PCR-RFLP. Figure 1 Photo 1 shows the gel electrophoresis results for the STAT6 rs324011 polymorphism. The lanes 2, 3, and 4 are heterozygous (CT) with three bands at 132, 107, & 25 bp. The lanes 1 and 5 were homozygous polymorphic (TT) with two bands at 107 & 25 bp. Figure 1 Photo 2 further validates these patterns, with lanes 2, 3, 4, and 5 were heterozygous (CT) with three bands at 132, 107, and 25 bp. Lane 1 was homozygous wild (CC) with a single band at 132 bp. The 25 bp band appears obscure due to its small molecular size
See this image and copyright information in PMC

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