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. 2025 Apr 1;17(4):e416-e421.
doi: 10.4317/jced.62010. eCollection 2025 Apr.

Histological effect of fluoride varnishes on teeth with caries in the white spot phase: An in vitro study

Margot Margarita Gutiérrez-Ilave  1 , Rocío Del Pilar Ríos-León  2 , Antonia Castro-Rodríguez  1 , María Rosario Calixto-Cotos  3
Affiliations

Histological effect of fluoride varnishes on teeth with caries in the white spot phase: An in vitro study

Margot Margarita Gutiérrez-Ilave et al. J Clin Exp Dent. .

Abstract

Background: The aim of this study was to compare the histological remineralization effects of two fluoride varnishes on artificially produced caries lesions in young permanent teeth.

Material and methods: Twenty longitudinal sections of premolars with artificially produced white spot lesions and evaluated with a polarised light microscope. The sample consisted of 20 tooth fragments, which were divided and assigned to two groups: The first group with application of Clinpro TM White Varnish (20 tooth sections) and the second, with MI Varnish TM (20 tooth sections) subjected for 6 days to conditions similar to the oral cavity. The sections were then re-evaluated by calculating the lesion depth and remineralization area (Image J software). Student' s t-test was used to analyse the data.

Results: MI Varnish TM fluoride varnish achieved a greater remineralization area than Clinpro TM White Varnish (838042.6±140359.3 μm2 and 678313.8±137265.7μm2, respectively), with a statistically significant difference (p<0.05).

Conclusions: MI Varnish TM had a better effect than Clinpro TM White Varnish in remineralizing lesions, in vitro, in the enamel of young permanent teeth with caries in the white-stained phase. Key words:Fluorides varnishes, histological effect, dental remineralization, polarised light microscope.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Remineralized areas by group (μm)2.
Figure 2
Figure 2
(A-E): Depth measurement with Image Pro Plus software (A) of demineralization ( B and D) and remineralization in enamel with Clinpro TM and Mi varnish TM ( C and E), respectively.
See this image and copyright information in PMC

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