A newly-isolated marine methanogen harbors a small cryptic plasmid
- PMID: 4037983
- DOI: 10.1007/BF00693400
A newly-isolated marine methanogen harbors a small cryptic plasmid
Abstract
Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a single AvaI site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci.