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. 2025:2900:145-159.
doi: 10.1007/978-1-0716-4398-3_9.

MicroRNA Profiling in Non-model Plants Using Microarray Hybridization

Luis Alfredo Cruz-Ramírez  1 , Christopher Cedillo-Jiménez  2 , Froylán Albarrán-Tamayo  3 , Bernardo Bañuelos-Hernández  3 , Sinue Isabel Morales-Alonso  3 , Andrés Cruz-Hernández  4
Affiliations

MicroRNA Profiling in Non-model Plants Using Microarray Hybridization

Luis Alfredo Cruz-Ramírez et al. Methods Mol Biol. 2025.

Abstract

The aim of this chapter is to describe how microarrays can be used to identify miRNAs that participate in fruit development in a non-model plant: prickly pear (Opuntia spp). Here, we describe a technology including molecular biology and bioinformatics methods combined to identify, select, and isolate candidate miRNAs putatively involved in prickly pear development. We describe how miRNA expression analysis through microarray hybridization was performed and the expression of selected miRNAs validated using RT-PCR.

Keywords: Hybridization; Microarrays; Prickly pear; RT-PCR Loop; miR156; miR157.

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