eDNA technology for monitoring terrestrial biodiversity: Technical highlights, challenges and progress
- PMID: 40380854
- DOI: 10.13287/j.1001-9332.202503.031
eDNA technology for monitoring terrestrial biodiversity: Technical highlights, challenges and progress
Abstract
Biodiversity is a fundamental prerequisite for human survival, constituting an indispensable component of global ecosystems. The United Nations Sustainable Development Goal 15 (SDG 15) underscores the significance of conserving terrestrial biodiversity and strives to advance the sustainable utilization of terrestrial ecosystems. To achieve this goal, it is of the utmost importance to establish a comprehensive biodiversity monitoring system. As an emerging monitoring tool, environmental DNA (eDNA) metabarcoding technology offers a number of advantages, including species morphology-independent, rapidity, economical, and high accuracy. Consequently, it provides an effective method for monitoring terrestrial biodiversity. We outlined the key technical aspects of using eDNA metabarcoding as a tool for biodiversity monitoring in terrestrial ecosystems, discussed the challenges of using eDNA metabarcoding technology in biodiversity research, along with strategies for addressing these challenges, surveyed recent research advances regarding eDNA from different sources for terrestrial biodiversity monitoring, and proposed future research directions.
生物多样性构成了人类生存的基础,是地球生态系统不可或缺的组成部分。联合国可持续发展目标15(SDG 15)强调了陆地生物多样性保护的重要性,旨在促进陆地生态系统的可持续利用。为了实现此目标,建立一个全面的生物多样性监测体系显得尤为关键。环境 DNA(environmental DNA,eDNA)宏条形码技术作为一种新兴的监测手段,具有不依赖于物种形态特征、快速、经济和高准确性等优势,为陆地生物多样性的监测提供了一种有效方法。本文首先总结了应用eDNA宏条形码技术进行陆地生态系统生物多样性监测的技术要点,重点探讨了当前eDNA宏条形码技术在生物多样性研究中面临的挑战及应对策略,同时梳理了不同来源eDNA在陆地生态系统生物多样性监测中的研究进展,最后对未来的研究方向进行了展望。.
Keywords: cross-domain biodiversity; eDNA source; metabarcoding primer; specific taxa; terrestrial ecosystem.
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