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Randomized Controlled Trial
. 2025 May 17;29(6):300.
doi: 10.1007/s00784-025-06374-6.

Clinical, tomographic, and histological analysis of post-extraction dental sockets filled with particulate dentin or blood clot: pilot study of a randomized clinical trial

Tobias Piton Fontana  1 , Pedro Henrique Corazza  1 , Dora Marise Medeiros de Castro  1 , Aline Bassani Deconto  1 , Letícia Copatti Dogenski  1 , Matheus Albino Souza  1 , Juliane Bervian  1 , Gisele Rovani  1 , Micheline Sandini Trentin  1 , João Paulo De Carli  2
Affiliations
Randomized Controlled Trial

Clinical, tomographic, and histological analysis of post-extraction dental sockets filled with particulate dentin or blood clot: pilot study of a randomized clinical trial

Tobias Piton Fontana et al. Clin Oral Investig. .

Abstract

Objective: This preliminary study aims to compare the clinical, radiological, and histological outcomes of third molar socket repairs after dental extraction with and without the use of particulate dentin graft.

Materials and methods: Ten patients requiring bilateral surgical extraction of impacted lower third molars were selected. One side received a blood clot (control side) and the other an autologous particulate dentin graft (experimental side). Postoperative evaluations at 7 and 21 days assessed pain, edema, trismus, suture dehiscence, and wound edge coaptation. At 120 days, CBCT was used to evaluate bone density, and a biopsy was performed for histological analysis. Data were analyzed using IBM SPSS software with descriptive statistics and paired t-test at a 5% significance level.

Results: At 21 days, only one case of suture dehiscence occurred on the experimental side, compared to three on the control side. No differences in pain, edema, or trismus were observed between groups. Tomographic analysis showed no significant difference in the distance from the alveolar bone crest to the CEJ, but the experimental group had significantly higher bone density (p = 0.002). Histologically, the experimental side exhibited thicker, denser connective tissue with higher cellularity, collagen production, and multinucleated giant cells, while the control side showed typical connective tissue with fewer multinucleated cells.

Conclusion: Particulate dentin grafting showed benefits in bone density and clinical stability, with a more advanced histological healing stage.

Clinical relevance: Improve post-extraction socket healing, offering a promising alternative to traditional methods, particularly in terms of enhancing bone regeneration and clinical outcomes.

Keywords: Alveolar bone graft; Biomaterials; Dentin; Third molar.

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Conflict of interest statement

Declarations. Ethical approval: This study was approved by the Research Ethics Committee of the University of Passo Fundo (CEP/UPF, approval no. 5.603.446) and registered in the Brazilian Clinical Trials Registry (ReBEC, registration no. RBR-4h6h9fd). Research involving human participants and/or animals: Approval was obtained from the ethics committee of University of Passo Fundo. The procedures used in this study adhere to the tenets of the Declaration of Helsinki. Informed consent: Patients invited to participate in the study signed a specific Free and Informed Consent Form. Competing interests: The authors declare no competing interests.

References

    1. Sammartino G, Tia M, Bucci T, Wang HL (2009) Prevention of mandibular third molar Extraction-Associated periodontal defects: A comparative study. J Periodontol 80(3):389–396 Doi: 10.1902/Jop.2009.080503 - DOI - PubMed
    1. Coleman M, Mccormick A, Laskin DM (2011) The incidence of periodontal defects distal to the maxillary second molar after impacted third molar extraction. J Oral Maxillofac Surg 69(2):319–321. https://doi.org/10.1016/J.Joms.2010.10.011 - DOI - PubMed
    1. Lee CT, Hum L, Chen YW (2016) The effect of regenerative periodontal therapy in preventing periodontal defects after the extraction of third molars: A systematic review and Meta-Analysis. J Am Dent Assoc 147(9):709–719E4. https://doi.org/10.1016/J.Adaj.2016.03.005 - DOI - PubMed
    1. De Biase A, Mazzucchi G, Di Nardo D, Lollobrigida M, Serafini G, Testarelli L (2020) Prevention of periodontal pocket formation after mandibular third molar extraction using dentin autologous graft: A split mouth case report. Case Rep Dent 2020:1762862. https://doi.org/10.1155/2020/1762862 - DOI - PubMed - PMC
    1. Hassan KS, Marei HF, Alagl AS (2012) Does grafting of third molar extraction sockets enhance periodontal measures in 30- to 35-Year-Old patients?? J Oral Maxillofac Surg 70(4):757–764. https://doi.org/10.1016/J.Joms.2011.09.010 - DOI - PubMed

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