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. 2025 Jun;15(6):172.
doi: 10.1007/s13205-025-04335-0. Epub 2025 May 16.

Identification and molecular characterization of a novel satellite associated with okra enation leaf curl virus infected okra (Abelmoschus esculentus L.) in India

Nishant  1 , Ramesh Kumar Yadav  1 , Damini Diksha  2 , Malyaj R Prajapati  2 , Harshwardhan Choudhary  1 , Suman Lata  1 , Mailem Y Shimray  2 , Reshav Naik  1 , Nitika Gupta  2 , Virendra Kumar Baranwal  2 , Susheel Kumar Sharma  2
Affiliations

Identification and molecular characterization of a novel satellite associated with okra enation leaf curl virus infected okra (Abelmoschus esculentus L.) in India

Nishant et al. 3 Biotech. 2025 Jun.

Abstract

Okra (Abelmoschus esculentus L.) is a major vegetable crop with significant commercial value, which suffers from yield losses due to various biotic stresses. Begomoviruses (family Geminiviridae) cause severe infection in okra in warmer regions worldwide. In 2023, a severe disease infection was observed in okra (Ablemoschus esculentus L.) at the research farm of ICAR-Indian Agriculture Research Institute, New Delhi, India. In this study a novel alphasatellite associated with the monopartite okra enation leaf curl virus (OELCuV) (Begomovirus abelsmoschusenation) begomovirus was identified by rolling circle amplification (RCA) in north India. Sequence analysis of OELCuV showed 88.98-99.02% nucleotide (nt) sequence identity with other OELCuV isolates. The alphasatellite shared 85.11% and 87.73% nt sequence identity with the symptomless alphasatellite associated with hollyhock yellow vein virus isolate Lahore 2 (NC_039084.1) and hollyhock yellow vein alphasatellite isolate J47 (MF683829.1), respectively. According to the species demarcation criteria based on the sequence similarity, it was concluded that it is a novel alphasatellite associated with OELCuV.

Keywords: Begomovirus abelsmoschusenation; India; Novel Alphasatellite; Okra; Okra enation leaf curl virus; RCA.

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Conflict of interest statement

Conflict of interestThe authors declare that the research was carried out without any commercial or financial ties that could be perceived as a potential conflict of interest.

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