Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb¹, Nadja Kampschulte¹, Gerhard Hagn², Kathrin Plitzko¹, Sven W Meckelmann³, Soumita Ghosh⁴, Robin Joshi⁴, Julia Kuligowski⁵, Dajana Vuckovic⁶, Marina T Botana^{2

7}, Ángel Sánchez-Illana⁸, Fereshteh Zandkarimi⁹, Aditi Das¹⁰, Jun Yang¹¹, Louis Schmidt¹², Antonio Checa², Helen M Roche^{13

14}, Aaron M Armando¹⁵, Matthew L Edin¹⁶, Fred B Lih¹⁶, Juan J Aristizabal-Henao¹⁷, Sayuri Miyamoto¹⁸, Francesca Giuffrida¹⁹, Arieh Moussaieff²⁰, Rosário Domingues²¹, Michael Rothe²², Christine Hinz²³, Ujjalkumar Subhash Das⁴, Katharina M Rund¹, Ameer Y Taha²⁴, Robert K Hofstetter²⁵, Markus Werner²⁵, Oliver Werz²⁵, Astrid S Kahnt²⁶, Justine Bertrand-Michel²⁷, Pauline Le Faouder²⁷, Robert Gurke^{28

29

30}, Dominique Thomas^{28

29

30}, Federico Torta^{31

32}, Ivana Milic³³, Irundika H K Dias³⁴, Corinne M Spickett³³, Denise Biagini³⁵, Tommaso Lomonaco³⁵, Helena Idborg³⁶, Jun-Yan Liu³⁷, Maria Fedorova³⁸, David A Ford³⁹, Anne Barden⁴⁰, Trevor A Mori⁴⁰, Paul D Kennedy⁴¹, Kirk Maxey⁴¹, Julijana Ivanisevic⁴², Hector Gallart-Ayala⁴², Cécile Gladine⁴³, Markus Wenk^{44

45}, Jean-Marie Galano⁴⁶, Thierry Durand⁴⁶, Ken D Stark⁴⁷, Coral Barbas⁴⁸, Ulrike Garscha¹², Stacy L Gelhaus^{49

50}, Uta Ceglarek⁵¹, Nicolas Flamand⁵², Julian L Griffin⁵³, Robert Ahrends⁵⁴, Makoto Arita⁵⁵, Darryl C Zeldin¹⁶, Francisco J Schopfer⁵⁰, Oswald Quehenberger¹⁵, Randall Julian⁵⁶, Anna Nicolaou⁵⁷, Ian A Blair⁵⁸, Michael P Murphy⁵⁹, Bruce D Hammock¹¹, Bruce Freeman⁵⁰, Gerhard Liebisch⁶⁰, Charles N Serhan⁶¹, Harald C Köfeler⁶², Per-Johan Jakobsson³⁶, Dieter Steinhilber²⁶, Michael H Gelb⁶³, Michal Holčapek⁶⁴, Ruth Andrew⁶⁵, Martin Giera⁶⁶, Garret A FitzGerald⁴, Robert C Murphy⁶⁷, John W Newman⁶⁸, Edward A Dennis¹⁵, Kim Ekroos⁶⁹, Ginger L Milne⁷⁰, Miguel A Gijón⁴¹, Hubert W Vesper⁷¹, Craig E Wheelock^{2

72}, Valerie B O'Donnell⁷³

Affiliations

¹ School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.
² Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
³ Applied Analytical Chemistry, University of Duisburg-Essen, Essen, Germany.
⁴ Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
⁵ Neonatal Research Group, Health Research Institute La Fe (IIS La Fe), Valencia, Spain.
⁶ Department of Chemistry and Biochemistry, Concordia University, Montreal, Canada.
⁷ School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
⁸ Department of Analytical Chemistry, University of Valencia, Burjassot, Spain.
⁹ Mass Spectrometry Core Facility, Department of Chemistry, Columbia University, New York, NY, USA.
¹⁰ School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
¹¹ Department of Entomology, University of California, Davis, CA, USA.
¹² Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Greifswald, Germany.
¹³ Nutrigenomics Research Group, UCD Conway Institute, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Ireland.
¹⁴ Institute for Global Food Security, Queen's University Belfast, Belfast, Northern Ireland.
¹⁵ Department of Pharmacology and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA.
¹⁶ Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC, USA.
¹⁷ BPG Bio, Framingham, MA, USA.
¹⁸ Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
¹⁹ Nestlé Institute of Food Safety and Analytical Sciences, Société des Produits Nestlé, Lausanne, Switzerland.
²⁰ Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
²¹ Department of Chemistry, University of Aveiro, Aveiro, Portugal.
²² Lipidomix GmbH, Berlin, Germany.
²³ Shimadzu UK Ltd., Milton Keynes, UK.
²⁴ Department of Food Science and Technology, University of California, Davis, CA, USA.
²⁵ Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University, Jena, Germany.
²⁶ Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.
²⁷ MetaboHUB-MetaToul, I2MC, Université de Toulouse, Inserm, Université Toulouse III-Paul Sabatier (UPS), Toulouse, France.
²⁸ Faculty of Medicine, Institute of Clinical Pharmacology, Goethe University Frankfurt, Frankfurt am Main, Germany.
²⁹ Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt am Main, Germany.
³⁰ Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD, Frankfurt am Main, Germany.
³¹ Signature Research Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore (NUS) Medical School, Singapore, Singapore.
³² Singapore Lipidomics Incubator, Life Sciences Institute and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
³³ School of Biosciences & Aston Institute for Membrane Excellence, Aston University, Aston Triangle, Birmingham, UK.
³⁴ Aston Medical School, College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, UK.
³⁵ Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, Italy.
³⁶ Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
³⁷ Center for Novel Target & Therapeutic Intervention, College of Pharmacy, Chongqing Medical University, Chongqing, P. R. China.
³⁸ Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany.
³⁹ Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University, St. Louis, MO, USA.
⁴⁰ Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, Australia.
⁴¹ Cayman Chemical Company, Ann Arbor, MI, USA.
⁴² Metabolomics and Lipidomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
⁴³ Université Clermont Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
⁴⁴ College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.
⁴⁵ Long Loo Lin School of Medicine, National University of Singapore.
⁴⁶ Institut des Biomolécules Max Mousseron (IBMM), Pôle Chimie Balard Recherche, CNRS, Université de Montpellier, ENSCN, Montpellier, France.
⁴⁷ Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Canada.
⁴⁸ Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, CEU San Pablo University, Madrid, Spain.
⁴⁹ Health Sciences Mass Spectrometry Core, University of Pittsburgh, Pittsburgh, PA, USA.
⁵⁰ Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
⁵¹ Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig Medical Center, Leipzig, Germany.
⁵² Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Département de médecine, Faculté de médecine, Université Laval, Québec City, Canada.
⁵³ Rowett Institute, School of Medicine, Medical Science and Nutrition, University of Aberdeen, Aberdeen, UK.
⁵⁴ Department of Analytical Chemistry, University of Vienna, Vienna, Austria.
⁵⁵ RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa, Japan.
⁵⁶ Indigo BioAutomation, Carmel, IN, USA.
⁵⁷ Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
⁵⁸ Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USA.
⁵⁹ Medical Research Council Mitochondrial Biology Unit and Department of Medicine, University of Cambridge, Cambridge, UK.
⁶⁰ Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany.
⁶¹ Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Hale Building for Transformative Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
⁶² Core Facility Mass Spectrometry, ZMF, Medical University of Graz, Graz, Austria.
⁶³ Department of Chemistry, University of Washington, Seattle, WA, USA.
⁶⁴ Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
⁶⁵ Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
⁶⁶ Leiden University Medical Center, Leiden, Netherlands.
⁶⁷ Department of Pharmacology, University of Colorado Denver, Denver, CO, USA.
⁶⁸ USDA ARS Western Human Nutrition Research Center and Department of Nutrition, University of California, Davis, CA, USA.
⁶⁹ Lipidomics Consulting Ltd., Esbo, Finland.
⁷⁰ Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Centre, Nashville, TN, USA.
⁷¹ Centers for Disease Control and Prevention, Atlanta, GA, USA.
⁷² Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, Sweden.
⁷³ School of Medicine, Cardiff University, Heath Park, Cardiff, UK.

PMID: 40392938
PMCID: PMC12330888
DOI: 10.1126/scisignal.adw1245

Review

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb et al. Sci Signal. 2025.

. 2025 May 20;18(887):eadw1245.

doi: 10.1126/scisignal.adw1245. Epub 2025 May 20.

Authors

Affiliations

¹ School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.
² Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
³ Applied Analytical Chemistry, University of Duisburg-Essen, Essen, Germany.
⁴ Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
⁵ Neonatal Research Group, Health Research Institute La Fe (IIS La Fe), Valencia, Spain.
⁶ Department of Chemistry and Biochemistry, Concordia University, Montreal, Canada.
⁷ School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
⁸ Department of Analytical Chemistry, University of Valencia, Burjassot, Spain.
⁹ Mass Spectrometry Core Facility, Department of Chemistry, Columbia University, New York, NY, USA.
¹⁰ School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
¹¹ Department of Entomology, University of California, Davis, CA, USA.
¹² Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Greifswald, Germany.
¹³ Nutrigenomics Research Group, UCD Conway Institute, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Ireland.
¹⁴ Institute for Global Food Security, Queen's University Belfast, Belfast, Northern Ireland.
¹⁵ Department of Pharmacology and Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA.
¹⁶ Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, Durham, NC, USA.
¹⁷ BPG Bio, Framingham, MA, USA.
¹⁸ Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
¹⁹ Nestlé Institute of Food Safety and Analytical Sciences, Société des Produits Nestlé, Lausanne, Switzerland.
²⁰ Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
²¹ Department of Chemistry, University of Aveiro, Aveiro, Portugal.
²² Lipidomix GmbH, Berlin, Germany.
²³ Shimadzu UK Ltd., Milton Keynes, UK.
²⁴ Department of Food Science and Technology, University of California, Davis, CA, USA.
²⁵ Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University, Jena, Germany.
²⁶ Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.
²⁷ MetaboHUB-MetaToul, I2MC, Université de Toulouse, Inserm, Université Toulouse III-Paul Sabatier (UPS), Toulouse, France.
²⁸ Faculty of Medicine, Institute of Clinical Pharmacology, Goethe University Frankfurt, Frankfurt am Main, Germany.
²⁹ Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Frankfurt am Main, Germany.
³⁰ Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD, Frankfurt am Main, Germany.
³¹ Signature Research Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore (NUS) Medical School, Singapore, Singapore.
³² Singapore Lipidomics Incubator, Life Sciences Institute and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
³³ School of Biosciences & Aston Institute for Membrane Excellence, Aston University, Aston Triangle, Birmingham, UK.
³⁴ Aston Medical School, College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, UK.
³⁵ Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, Italy.
³⁶ Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
³⁷ Center for Novel Target & Therapeutic Intervention, College of Pharmacy, Chongqing Medical University, Chongqing, P. R. China.
³⁸ Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany.
³⁹ Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University, St. Louis, MO, USA.
⁴⁰ Medical School, Royal Perth Hospital Unit, University of Western Australia, Perth, Australia.
⁴¹ Cayman Chemical Company, Ann Arbor, MI, USA.
⁴² Metabolomics and Lipidomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
⁴³ Université Clermont Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
⁴⁴ College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.
⁴⁵ Long Loo Lin School of Medicine, National University of Singapore.
⁴⁶ Institut des Biomolécules Max Mousseron (IBMM), Pôle Chimie Balard Recherche, CNRS, Université de Montpellier, ENSCN, Montpellier, France.
⁴⁷ Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Canada.
⁴⁸ Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, CEU San Pablo University, Madrid, Spain.
⁴⁹ Health Sciences Mass Spectrometry Core, University of Pittsburgh, Pittsburgh, PA, USA.
⁵⁰ Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
⁵¹ Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig Medical Center, Leipzig, Germany.
⁵² Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Département de médecine, Faculté de médecine, Université Laval, Québec City, Canada.
⁵³ Rowett Institute, School of Medicine, Medical Science and Nutrition, University of Aberdeen, Aberdeen, UK.
⁵⁴ Department of Analytical Chemistry, University of Vienna, Vienna, Austria.
⁵⁵ RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa, Japan.
⁵⁶ Indigo BioAutomation, Carmel, IN, USA.
⁵⁷ Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
⁵⁸ Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USA.
⁵⁹ Medical Research Council Mitochondrial Biology Unit and Department of Medicine, University of Cambridge, Cambridge, UK.
⁶⁰ Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany.
⁶¹ Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Hale Building for Transformative Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
⁶² Core Facility Mass Spectrometry, ZMF, Medical University of Graz, Graz, Austria.
⁶³ Department of Chemistry, University of Washington, Seattle, WA, USA.
⁶⁴ Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
⁶⁵ Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
⁶⁶ Leiden University Medical Center, Leiden, Netherlands.
⁶⁷ Department of Pharmacology, University of Colorado Denver, Denver, CO, USA.
⁶⁸ USDA ARS Western Human Nutrition Research Center and Department of Nutrition, University of California, Davis, CA, USA.
⁶⁹ Lipidomics Consulting Ltd., Esbo, Finland.
⁷⁰ Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Centre, Nashville, TN, USA.
⁷¹ Centers for Disease Control and Prevention, Atlanta, GA, USA.
⁷² Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, Sweden.
⁷³ School of Medicine, Cardiff University, Heath Park, Cardiff, UK.

PMID: 40392938
PMCID: PMC12330888
DOI: 10.1126/scisignal.adw1245

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

PubMed Disclaimer

Conflict of interest statement

Competing financial interests

VBD is a consultant for Metasight. BDH is a founder of EicOsis Human Health with a potential pharmaceutical in clinical trials whose action depends on altering the profile of inflammatory lipids. MG is a cofounder of the company Enfanos, LLC. BF acknowledges a financial interest in Creegh Pharmaceuticals Inc.. EAD is a cofounder of LipoNexus, Inc..

References

1. Ricciotti E, FitzGerald GA, Aspirin in the Prevention of Cardiovascular Disease and Cancer. Annu Rev Med 72, 473–495 (2021); published online EpubJan 27 ( 10.1146/annurev-med-051019-102940). - DOI - PubMed
1. von Euler US, On the specific vasodilating and plain muscle stimulating substances from accessory genital glands in man and certain animals (prostaglandin and vesigIandin). J. Physiol. (Lond. 88, 213 (1936). - PMC - PubMed
1. Bergstroem S, Danielsson H, Samuelsson B, The Enzymatic Formation of Prostaglandin E2 from Arachidonic Acid Prostaglandins and Related Factors 32. Biochim Biophys Acta 90, 207–210 (1964); published online EpubJul 15 ( 10.1016/0304-4165(64)90145-x). - DOI - PubMed
1. Vane JR, Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol 231, 232–235 (1971); published online EpubJun 23 ( 10.1038/newbio231232a0). - DOI - PubMed
1. Fahy E, Subramaniam S, Brown HA, Glass CK, Merrill AH Jr., Murphy RC, Raetz CR, Russell DW, Seyama Y, Shaw W, Shimizu T, Spener F, van Meer G, VanNieuwenhze MS, White SH, Witztum JL, Dennis EA, A comprehensive classification system for lipids. J Lipid Res 46, 839–861 (2005); published online EpubMay ( 10.1194/jlr.E400004-JLR200). - DOI - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Affiliations

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Authors

Affiliations

Abstract

Conflict of interest statement

References

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources