doi: 10.1016/0005-2787(77)90286-6.
Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation studies
- PMID: 403949
- DOI: 10.1016/0005-2787(77)90286-6
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Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation studies
Biochim Biophys Acta.
.
Abstract
It is shown in this work that the binding of ethidium bromide to yeast ribosomes occurs through intercalation in the double-stranded rRNA regions and produces changes in the ribosomes structure, yielding unfolded subparticles, and even partial separation of proteins from rRNA at very high binding ratios. The addition of Mg2+ prevents these structural changes, probably by partial inhibition of the dye binding.
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